Debnath J C, Mallick B B, Das S K
Immunostimulation Laboratory, Indian Veterinary Research Institute, Izatnagar.
Indian J Exp Biol. 1992 Feb;30(2):73-6.
On inoculation of nonspecific stimulator of immunity (NSI), prepared from Mycobacterium phlei (M. phlei), simultaneously along with sheep pox virus (SPV) in sheep, the recipient has exhibited appreciable level of SPV specific antibody as early as on 10th day which reached at peak level on 20th day and remained unaltered on 30th day of postimmunisation as evinced by serum neutralisation test (SNT), enzyme linked immunosorbant assay (ELISA) indirect, fluorescent antibody technique (FAT) indirect, counter immunoelectrophoresis (CIEP) and finally by virulent SPV challenge. On the contrary, sheep, when immunised with SPV only could not produce appreciable level of antibody on 10th day but did so on 20th day of inoculation. SPV and NSI immunised sheep produced enhanced protection against virulent SPV challenge in comparison with sheep immunised with SPV only. Healthy control sheep, however, could not resist challenge.
在用从草分枝杆菌制备的非特异性免疫刺激剂（NSI）与绵羊痘病毒（SPV）同时接种绵羊时，受体早在第10天就表现出相当水平的SPV特异性抗体，在第20天达到峰值水平，并在免疫后第30天保持不变，血清中和试验（SNT）、间接酶联免疫吸附测定（ELISA）、间接荧光抗体技术（FAT）、对流免疫电泳（CIEP）以及最后通过强毒SPV攻击均证明了这一点。相反，仅用SPV免疫的绵羊在第10天不能产生相当水平的抗体，但在接种后第20天能产生。与仅用SPV免疫的绵羊相比，用SPV和NSI免疫的绵羊对强毒SPV攻击产生了更强的保护作用。然而，健康对照绵羊无法抵抗攻击。
