Sharma B, Negi B S, Yadav M P, Shankar H, Pandey A B
Division of Virology, Indian Veterinary Research Institute, Mukteswar-Kumaon (UP), India.
Acta Virol. 1988 Jan;32(1):65-9.
Enzyme linked immunosorbent assay (ELISA) was standardized for detection of goat pox virus (GPV) antibodies and antigen using live and inactivated antigens and hyperimmune serum (HIS), convalescent, post-vaccinal, as also post-challenge sera. The ELISA was most sensitive in detection of antibody when compared with agar gel precipitation (AGP) and counterimmunoelectrophoresis (CIE) tests. There was no complete correlation between the antibody status of vaccinated goats and protective immunity as animals having detectable seroconversion were also solidly immune to virulent challenge. The application of ELISA in pox infections of goats has been discussed.
酶联免疫吸附测定（ELISA）已标准化，用于使用活抗原、灭活抗原、超免疫血清（HIS）、恢复期血清、疫苗接种后血清以及攻毒后血清检测山羊痘病毒（GPV）抗体和抗原。与琼脂凝胶沉淀（AGP）和对流免疫电泳（CIE）试验相比，ELISA在检测抗体方面最为敏感。接种疫苗的山羊的抗体状态与保护性免疫之间没有完全的相关性，因为血清转化可检测到的动物对强毒攻击也具有牢固的免疫力。本文讨论了ELISA在山羊痘感染中的应用。
