Iwasaka T, Yokoyama M, Oh-uchida M, Matsuo N, Hara K, Fukuyama K, Hachisuga T, Fukuda K, Sugimori H
Department of Obstetrics and Gynecology, Saga Medical School, Japan.
Gynecol Oncol. 1992 Sep;46(3):298-303. doi: 10.1016/0090-8258(92)90220-d.
Invasive carcinomas of the uterine cervix of 38 patients were examined for the presence of human papillomavirus (HPV) genomes and for the state of the c-myc and Ha-ras oncogenes. A combination of Southern blot hybridization and polymerase chain reaction revealed the presence of the genome of HPV type 16 in 17 tumors (45%), that of HPV type 18 in 3 tumors (8%), and that of unknown types in 16 others (42%), while no viral DNA sequences were detected in 2 tumors. Of the 38 tumors, c-myc amplification was found in only 1 tumor, while there was no Ha-ras amplification. Overexpression of the c-myc gene was observed in 15 (44%) of the 34 tumors analyzed, while there was no overexpression of Ha-ras. Of the 23 squamous cell carcinomas analyzed, relapse-free rates at 24 months were 55% in tumors with c-myc overexpression and 100% in case of tumors with no c-myc overexpression, respectively. The results suggest the possibility that activation of the c-myc oncogene is involved in tumor progression.
对38例子宫颈浸润癌患者的肿瘤组织进行检测，分析人乳头瘤病毒（HPV）基因组的存在情况以及c-myc和Ha-ras癌基因的状态。采用Southern印迹杂交和聚合酶链反应相结合的方法，结果显示17例肿瘤（45%）中存在16型HPV基因组，3例肿瘤（8%）中存在18型HPV基因组，16例肿瘤（42%）中存在未知型HPV基因组，2例肿瘤未检测到病毒DNA序列。在38例肿瘤中，仅1例肿瘤检测到c-myc基因扩增，未检测到Ha-ras基因扩增。在34例分析的肿瘤中，15例（44%）观察到c-myc基因过表达，未观察到Ha-ras基因过表达。在23例分析的鳞状细胞癌中，c-myc基因过表达的肿瘤24个月无复发生存率为55%，c-myc基因未过表达的肿瘤24个月无复发生存率为100%。结果提示c-myc癌基因的激活可能参与肿瘤进展。
