Macri C I, Cook N S, Walker J L, Berman M L, Patton T J, Wilczynski S P
Department of Obstetrics and Gynecology, University of California, Irvine Medical Center, Orange.
Gynecol Oncol. 1992 Sep;46(3):372-6. doi: 10.1016/0090-8258(92)90235-b.
Human papillomavirus (HPV) DNA has been shown by molecular hybridization studies to persist in both recurrent and metastatic disease in tumors of the female genital tract. We report here the use of the polymerase chain reaction to identify HPV DNA in material from fine-needle aspirates (FNA) of recurrent or metastatic lesions to document the primary malignancy arising in the lower genital tract. Fine-needle aspirates of suspected recurrent or metastatic tumors were obtained from nine patients with carcinoma of the lower genital tract and five patients with malignancies that have not been associated with HPV. DNA was extracted from the FNA and tissue block, when available, and amplified with HPV 6, HPV 16, and HPV 18 specific primers. In eight of the nine tumors from the lower genital tract, HPV DNA was identified in both the primary and metastatic lesions. In every case the HPV genotype was identical. One cervical carcinoma and five non-HPV associated tumors were negative for papillomavirus DNA. This study demonstrates that molecular hybridization techniques can be useful in identifying the source of a metastasis and have the potential to diagnose the presence of metastatic disease by detecting HPV DNA even when the cytologic criteria are equivocal.
分子杂交研究显示，人乳头瘤病毒（HPV）DNA在女性生殖道肿瘤的复发性和转移性疾病中持续存在。我们在此报告使用聚合酶链反应来鉴定来自复发性或转移性病变细针穿刺抽吸物（FNA）中的HPV DNA，以证明下生殖道原发性恶性肿瘤的存在。从9名下生殖道癌患者和5名与HPV无关的恶性肿瘤患者中获取疑似复发性或转移性肿瘤的细针穿刺抽吸物。如有可用的组织块，则从FNA和组织块中提取DNA，并用HPV 6、HPV 16和HPV 18特异性引物进行扩增。在9名下生殖道肿瘤中的8例中，原发性和转移性病变均检测到HPV DNA。在每种情况下，HPV基因型均相同。1例宫颈癌和5例与HPV无关的肿瘤乳头瘤病毒DNA检测呈阴性。这项研究表明，分子杂交技术可用于确定转移灶的来源，并且即使在细胞学标准不明确时，也有可能通过检测HPV DNA来诊断转移性疾病的存在。
