Whalen M M, Doshi R N, Bankhurst A D
Department of Medicine, University of New Mexico School of Medicine, Albuquerque 87131.
Immunology. 1992 Jul;76(3):402-7.
Membranes from highly purified natural killer (NK) cells were ADP ribosylated by treatment with pertussis toxin (PTX). PTX treatment resulted in a single band of 32P incorporation at M(r) 41,600. PTX treatment of NK cells diminished their ability to lyse K562 tumour cells by about 50%. However PTX treatment had no measurable effect on cAMP levels in NK cells. PTX pretreatment also had no effect on the ability of target cells to induce phosphoinositide turnover or on the ability of the NK cells to conjugate with the K562 tumour cells. Movement toward the chemoattractants interleukin-2 (IL-2) and formylmethionylleucylphenylalanine (FMLP) was significantly inhibited indicating that a PTX substrate in NK cells may be involved in the transduction of signals which are involved in cell motility.
用百日咳毒素（PTX）处理来自高度纯化的自然杀伤（NK）细胞的膜，可使其发生ADP核糖基化。PTX处理导致在分子量为41,600处出现一条32P掺入带。PTX处理NK细胞使其裂解K562肿瘤细胞的能力降低约50%。然而，PTX处理对NK细胞中的cAMP水平没有可测量的影响。PTX预处理对靶细胞诱导磷酸肌醇周转的能力或NK细胞与K562肿瘤细胞结合的能力也没有影响。向趋化因子白细胞介素-2（IL-2）和甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）的移动受到显著抑制，这表明NK细胞中的一种PTX底物可能参与了与细胞运动相关的信号转导。
