Van Epps D E, Durant D A, Potter J W
J Immunol. 1983 Aug;131(2):697-700.
Previous studies have shown that supernatants from Con A-stimulated human mononuclear cells are chemotactic for T cells, and that the source of the activity is the Leu-2 (suppressor/cytotoxic) T cell. The specificity of this chemoattractant activity for Leu-2 and/or Leu-3 (helper/inducer) T cells was analyzed with isolated human T cell subsets in an in vitro chemotaxis assay system. These studies demonstrate that both human Leu-2 and Leu-3 cells migrate in response to the lymphocyte locomotor stimulus casein, whereas only Leu-3 cells migrate in response to supernatants from Con A-stimulated mononuclear cells. This migration of Leu-3 cells to the Con A supernatant was reflected in both the distance migrated and the number of migrating cells. When Leu-2 cell locomotion in response to Con A supernatants was analyzed, no significant differences from control were observed in either the distance migrated or the number of migrating cells. Supernatants from Con A-stimulated Leu-2 cells similarly attracted Leu-3 cells but not Leu-2 cells in a concentration-dependent manner. Supernatants from Con A-stimulated Leu-3 cells did not stimulate either Leu-2 or Leu-3 cell migration. These studies demonstrate that Leu-3 T cells migrate in response to supernatants from Con A-stimulated Leu-2 T cells. This specific response may promote cell-to-cell interaction by providing a means for Leu-2 T cells to recruit Leu-3 T cells.
先前的研究表明，刀豆蛋白A刺激的人单核细胞的上清液对T细胞具有趋化作用，且该活性来源是Leu-2（抑制/细胞毒性）T细胞。在体外趋化分析系统中，用分离的人T细胞亚群分析了这种趋化活性对Leu-2和/或Leu-3（辅助/诱导）T细胞的特异性。这些研究表明，人Leu-2和Leu-3细胞均对淋巴细胞运动刺激物酪蛋白产生迁移反应，而只有Leu-3细胞对刀豆蛋白A刺激的单核细胞的上清液产生迁移反应。Leu-3细胞向刀豆蛋白A上清液的这种迁移在迁移距离和迁移细胞数量上均有体现。当分析Leu-2细胞对刀豆蛋白A上清液的运动时，在迁移距离或迁移细胞数量上均未观察到与对照有显著差异。刀豆蛋白A刺激的Leu-2细胞的上清液同样以浓度依赖的方式吸引Leu-3细胞，但不吸引Leu-2细胞。刀豆蛋白A刺激的Leu-3细胞的上清液既不刺激Leu-2细胞也不刺激Leu-3细胞迁移。这些研究表明，Leu-3 T细胞对刀豆蛋白A刺激的Leu-2 T细胞的上清液产生迁移反应。这种特异性反应可能通过为Leu-2 T细胞募集Leu-3 T细胞提供一种方式来促进细胞间相互作用。
