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两种细胞单链特异性DNA结合蛋白与牛乳头瘤病毒1型基因组的两个区域相互作用，包括DNA复制起点。

Two cellular single-strand-specific DNA-binding proteins interact with two regions of the bovine papillomavirus type 1 genome, including the origin of DNA replication.

作者信息

Habiger C, Stelzer G, Schwarz U, Winnacker E L

机构信息

Institut für Biochemie, Universität München im Max-Planck-Institut für Biochemie, Germany.

出版信息

J Virol. 1992 Oct;66(10):5988-98. doi: 10.1128/JVI.66.10.5988-5998.1992.

DOI:10.1128/JVI.66.10.5988-5998.1992
PMID:1326653
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC241476/
Abstract

We have identified and purified to near homogeneity two specific single-stranded DNA-binding factors (SPSF I and II) with molecular masses of 42 and 39 kDa, respectively, from calf thymus. Gel retention analysis and competition experiments demonstrate that the ubiquitous proteins SPSF I and II specifically interact with single-stranded DNA derived from the minimal in vitro origin of replication of bovine papillomavirus type 1 and a region of the viral genome proposed to be involved in plasmid maintenance. Bovine papillomavirus type 1 proteins do not interfere with DNA binding of SPSF I and II. The exact location of the binding domains of SPSF I and II on the DNA has been determined by methylation interference and T4 DNA polymerase footprinting. A potential cellular binding site for SPSF I and II is the major promoter (P2) of the human c-myc gene.

摘要

我们从小牛胸腺中鉴定并纯化出了两种特异性单链DNA结合因子（SPSF I和II），其分子量分别为42 kDa和39 kDa，纯度接近均一。凝胶滞留分析和竞争实验表明，普遍存在的蛋白质SPSF I和II与源自牛乳头瘤病毒1型体外最小复制起点的单链DNA以及病毒基因组中一个被认为与质粒维持有关的区域特异性相互作用。牛乳头瘤病毒1型蛋白不干扰SPSF I和II与DNA的结合。通过甲基化干扰和T4 DNA聚合酶足迹法确定了SPSF I和II在DNA上结合结构域的确切位置。SPSF I和II潜在的细胞结合位点是人c-myc基因的主要启动子（P2）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9266/241476/140c4139ff1c/jvirol00041-0302-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9266/241476/bdb743bf3e98/jvirol00041-0296-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9266/241476/8dad7f9b6741/jvirol00041-0296-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9266/241476/d50a211bc8e0/jvirol00041-0297-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9266/241476/fcd4494f368b/jvirol00041-0298-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9266/241476/3139aebf5164/jvirol00041-0299-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9266/241476/32725e60f1c5/jvirol00041-0299-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9266/241476/9a08aaabc96d/jvirol00041-0300-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9266/241476/7774a6b049dc/jvirol00041-0301-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9266/241476/140c4139ff1c/jvirol00041-0302-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9266/241476/bdb743bf3e98/jvirol00041-0296-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9266/241476/8dad7f9b6741/jvirol00041-0296-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9266/241476/d50a211bc8e0/jvirol00041-0297-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9266/241476/fcd4494f368b/jvirol00041-0298-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9266/241476/3139aebf5164/jvirol00041-0299-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9266/241476/32725e60f1c5/jvirol00041-0299-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9266/241476/9a08aaabc96d/jvirol00041-0300-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9266/241476/7774a6b049dc/jvirol00041-0301-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9266/241476/140c4139ff1c/jvirol00041-0302-a.jpg

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引用本文的文献

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Characterization of the single-strand-specific BPV-1 origin binding protein, SPSF I, as the HeLa Pur alpha factor.单链特异性牛乳头瘤病毒1型（BPV-1）起始结合蛋白SPSF I作为海拉细胞Purα因子的特性分析
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2
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J Virol. 1995 Feb;69(2):651-60. doi: 10.1128/JVI.69.2.651-660.1995.

本文引用的文献

1
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.从分离的哺乳动物细胞核的可溶性提取物中，RNA聚合酶II进行准确的转录起始。
Nucleic Acids Res. 1983 Mar 11;11(5):1475-89. doi: 10.1093/nar/11.5.1475.
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Rapid and quantitative recovery of DNA fragments from gels by displacement electrophoresis (isotachophoresis).通过置换电泳（等速电泳）从凝胶中快速定量回收DNA片段。
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Chromatin structure and protein binding in the putative regulatory region of the c-myc gene in Burkitt lymphoma.
伯基特淋巴瘤中c-myc基因假定调控区域的染色质结构与蛋白质结合
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An enhancer sequence from bovine papilloma virus DNA consists of two essential regions.来自牛乳头瘤病毒DNA的增强子序列由两个必需区域组成。
Nucleic Acids Res. 1984 Mar 26;12(6):2901-16. doi: 10.1093/nar/12.6.2901.
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Characterization of the bovine papilloma virus plasmid maintenance sequences.牛乳头瘤病毒质粒维持序列的特征分析
Cell. 1984 Feb;36(2):391-401. doi: 10.1016/0092-8674(84)90232-0.
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Bovine papilloma virus contains an activator of gene expression at the distal end of the early transcription unit.牛乳头瘤病毒在早期转录单元的远端含有一个基因表达激活剂。
Mol Cell Biol. 1983 Jun;3(6):1108-22. doi: 10.1128/mcb.3.6.1108-1122.1983.
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Isolation of episomal bovine papillomavirus chromatin and identification of a DNase I-hypersensitive region.游离型牛乳头瘤病毒染色质的分离及核酸酶 I 超敏区域的鉴定。
J Virol. 1983 May;46(2):567-74. doi: 10.1128/JVI.46.2.567-574.1983.
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Transformation and replication in mouse cells of a bovine papillomavirus--pML2 plasmid vector that can be rescued in bacteria.一种可在细菌中拯救的牛乳头瘤病毒-pML2质粒载体在小鼠细胞中的转化与复制。
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7147-51. doi: 10.1073/pnas.79.23.7147.
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Mouse cells transformed by bovine papillomavirus contain only extrachromosomal viral DNA sequences.被牛乳头瘤病毒转化的小鼠细胞仅含有染色体外的病毒DNA序列。
Proc Natl Acad Sci U S A. 1981 May;78(5):2727-31. doi: 10.1073/pnas.78.5.2727.
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In vitro tumorigenic transformation by a defined sub-genomic fragment of bovine papilloma virus DNA.牛乳头瘤病毒DNA特定亚基因组片段的体外致瘤转化
Nature. 1980 Sep 4;287(5777):72-4. doi: 10.1038/287072a0.