Tanikawa E, Mori O, Hachisuka H, Sasai Y
Department of Dermatology, Kurume University School of Medicine, Japan.
Acta Histochem. 1992;93(1):282-9. doi: 10.1016/S0065-1281(11)80225-4.
The cellular proto-oncogene, ras, is known to play an important role in the regulation of cell growth and proliferation in normal and malignant conditions. The present study was undertaken to immunohistochemically examine the expression of ras protooncogene product p21 in normal human skin and some cutaneous tumours. In normal skin, the expression of p21 was found in sweat glands, sebaceous glands, capillary endothelium, and smooth muscles, while epidermis was devoid of reaction product. Keratoacanthoma and the granular cells of verruca vulgaris were immunoreactive to the antibody for p21. Bowen's disease and squamous cell carcinoma were positive for p21, but basal cell carcinoma and seborrheic keratosis were negative. In mammary and extramammary Paget's diseases, the immunoreactivity was inconsistent. The expression of p21 in malignant melanoma cells was intense, whereas normal melanocytes and nevus cells were devoid of the expression. These results suggest that the expression of p21 does not correlate with nuclear anaplasia and malignant behaviour of cutaneous tumours.
细胞原癌基因ras在正常和恶性条件下的细胞生长和增殖调控中起着重要作用。本研究旨在通过免疫组织化学方法检测ras原癌基因产物p21在正常人体皮肤和一些皮肤肿瘤中的表达。在正常皮肤中，p21的表达见于汗腺、皮脂腺、毛细血管内皮和平滑肌，而表皮无反应产物。角化棘皮瘤和寻常疣的颗粒细胞对p21抗体呈免疫反应性。鲍温病和鳞状细胞癌p21呈阳性，但基底细胞癌和脂溢性角化病呈阴性。在乳腺和乳腺外佩吉特病中，免疫反应性不一致。恶性黑色素瘤细胞中p21的表达强烈，而正常黑素细胞和痣细胞无此表达。这些结果表明，p21的表达与皮肤肿瘤的核间变和恶性行为无关。
