Department of Pathology and Dermatology, College of Medicine, Hallym University, Anyang, Korea.
Int J Dermatol. 2010 Jan;49(1):48-52. doi: 10.1111/j.1365-4632.2009.04191.x.
Ezrin is a cytoskeleton linker protein that is actively involved in regulating the growth and metastatic capacity of cancer cells. The purpose of the study was to assess the expression pattern of ezrin in normal skin and various epithelial neoplasms.
We used immunohistochemical techniques to examine the expression of ezrin in paraffin-embedded tissues of squamous cell carcinoma (n = 23), basal cell carcinoma (n = 10), Bowen's disease (n = 10), actinic keratosis (n = 10), keratoacanthoma (n = 9), seborrheic keratosis (n = 5), psoriasis vulgaris (n = 5), and normal control skin (n = 5).
In Bowen's disease, actinic keratosis, keratoacanthoma, and seborrheic keratosis, ezrin was dominantly expressed in the cell membrane except for the cornified layer. In squamous cell carcinoma (SCC) specimens, the percentage of ezrin-positive cells was increased compared with Bowen's disease, actinic keratosis, keratoacanthoma, and seborrheic keratosis. Especially in SCC samples, ezrin expression was markedly expressed in the cytoplasm. In addition, there was a correlation between the pattern of ezrin expression and tumor differentiation in SCC. Basal cell carcinoma showed intense and diffuse staining especially in the solid growth pattern.
Our findings suggest that dysregulation of ezrin may be important in the development of cutaneous epithelial malignancies and tumor grade. We suggest that the cytoplasmic localization of ezrin may be useful in the diagnosis of skin SCC.
Ezrin 是一种细胞骨架连接蛋白，它积极参与调节癌细胞的生长和转移能力。本研究的目的是评估 Ezrin 在正常皮肤和各种上皮肿瘤中的表达模式。
我们使用免疫组织化学技术检测了 23 例鳞状细胞癌、10 例基底细胞癌、10 例 Bowen 病、10 例光化性角化病、9 例角化棘皮瘤、5 例脂溢性角化病、5 例寻常型银屑病和 5 例正常对照皮肤组织中 Ezrin 的表达。
在 Bowen 病、光化性角化病、角化棘皮瘤和脂溢性角化病中，Ezrin 除角化层外主要表达于细胞膜。与 Bowen 病、光化性角化病、角化棘皮瘤和脂溢性角化病相比，鳞状细胞癌（SCC）标本中 Ezrin 阳性细胞的比例增加。特别是在 SCC 样本中，Ezrin 表达在细胞质中明显表达。此外，Ezrin 表达模式与 SCC 的肿瘤分化之间存在相关性。基底细胞癌表现为强烈且弥漫的染色，特别是在实体生长模式中。
我们的研究结果表明，Ezrin 的失调可能在皮肤上皮恶性肿瘤和肿瘤分级的发展中很重要。我们认为 Ezrin 的细胞质定位可能有助于 SCC 的诊断。
