Ohman L, Dahlgren C, Follin P, Lew D, Stendahl O
Department of Microbiology, Immunology and Infectious Diseases, Faculty of Health Sciences, Linköping University, Sweden.
Agents Actions. 1992 Jul;36(3-4):271-7.
The effect of the sulphur compound N-acetylcysteine (NAC) on certain receptor-mediated cellular functions [chemiluminescence (CL), phagocytosis and degranulation] in human neutrophils was studied, to evaluate how a scavenger of certain toxic oxygen product can protect the phagocyte and the bystander tissue cells from oxidative damage. When using IgG-opsonized yeast particles as stimulating agent, preincubating the neutrophils with NAC (0.25 mg/ml = 1.5 mM) increased both the CL response and phagocytosis. Higher concentrations of NAC (0.50-1.00 mg/ml = 3-6 mM) decreased the CL response, whereas the phagocytic capacity was still enhanced. This effect was more pronounced with adherent neutrophils than with neutrophils in suspension. No increased CL or phagocytic activity was, however, induced by NAC when C3bi-opsonized particles were used as a prey. From the fact that NAC (i) inhibited extracellularly localized myeloperoxidase dependent activities, and (ii) had no effect on neutrophils from patients with chronic granulomatous disease (CGD), we conclude that the scavenger effect of NAC not only reduces the accumulation of oxidative metabolites per se, but also enhances receptor-mediated phagocytosis by protecting Fc(IgG)-receptors from oxidative damage mediated by myeloperoxidase (MPO) and hydrogen peroxide (H2O2). Since NAC can increase phagocytosis and reduce the extracellularly produced oxidative metabolites, we furthermore conclude that NAC possesses some ideal properties as an anti-inflammatory agent.
研究了硫化合物N - 乙酰半胱氨酸（NAC）对人中性粒细胞中某些受体介导的细胞功能[化学发光（CL）、吞噬作用和脱颗粒作用]的影响，以评估某种有毒氧产物的清除剂如何保护吞噬细胞和旁观者组织细胞免受氧化损伤。当使用IgG调理的酵母颗粒作为刺激剂时，用NAC（0.25 mg/ml = 1.5 mM）预孵育中性粒细胞可增加CL反应和吞噬作用。更高浓度的NAC（0.50 - 1.00 mg/ml = 3 - 6 mM）会降低CL反应，而吞噬能力仍会增强。与悬浮的中性粒细胞相比，贴壁中性粒细胞的这种效应更明显。然而，当使用C3bi调理的颗粒作为猎物时，NAC不会诱导CL或吞噬活性增加。基于NAC（i）在细胞外抑制髓过氧化物酶依赖性活性，以及（ii）对慢性肉芽肿病（CGD）患者的中性粒细胞无影响这一事实，我们得出结论，NAC的清除剂作用不仅能减少氧化代谢产物本身的积累，还能通过保护Fc（IgG）受体免受髓过氧化物酶（MPO）和过氧化氢（H2O2）介导的氧化损伤来增强受体介导的吞噬作用。由于NAC可以增加吞噬作用并减少细胞外产生的氧化代谢产物，我们进一步得出结论，NAC作为一种抗炎剂具有一些理想的特性。
