两种含硫醇药物对人中性粒细胞过氧化氢生成的体外作用比较。

Comparison of in vitro effects of two thiol-containing drugs on human neutrophils hydrogen peroxide production.

作者信息

Gressier B, Cabanis A, Lebegue S, Brunet C, Dine T, Luyckx M, Cazin M, Cazin J C

机构信息

Faculté des Sciences Pharmaceutiques et Biologiques, Laboratoire de Pharmacologie, Pharmacocinétique et Pharmacie Clinique, Lille, France.

出版信息

Methods Find Exp Clin Pharmacol. 1993 Mar;15(2):101-5.

PMID:8487592

Abstract

During inflammatory disorders, potentially destructive reactive oxygen species, especially hydrogen peroxide, are produced by activated phagocytic cells. It was demonstrated in vitro that mesna and N-acetylcysteine (NAC), mucolytic thiols, have antioxidant properties. An estimation was made of the 50% inhibitory concentration (IC50) of mesna and NAC for PMA-induced H2O2 production by human neutrophils, the results being 70 mcM and 77 mcM, respectively. The mechanism which governs mesna and NAC reactions results from a scavenging effect of H2O2: the calculated IC50s of this effect were 30 mcM and 42 mcM, respectively, in free cellular experimentation. The results suggest that mesna and NAC might be used as antioxidants in aerosols to prevent tissue damage inflicted by this reactive oxygen species, especially in the lungs.

摘要

在炎症性疾病期间，活化的吞噬细胞会产生具有潜在破坏性的活性氧物质，尤其是过氧化氢。体外实验表明，溶黏蛋白的美司钠和N - 乙酰半胱氨酸（NAC）具有抗氧化特性。测定了美司钠和NAC对人中性粒细胞经佛波酯（PMA）诱导产生过氧化氢的50%抑制浓度（IC50），结果分别为70 μM和77 μM。美司钠和NAC反应的机制源于对过氧化氢的清除作用：在游离细胞实验中，这种清除作用的计算IC50分别为30 μM和42 μM。结果表明，美司钠和NAC可用作气雾剂中的抗氧化剂，以防止这种活性氧物质造成的组织损伤，尤其是在肺部。