Foglesong P D, Reckord C
Biology Department, Rutgers University, Camden, NJ 08102.
Biotechniques. 1992 Sep;13(3):402-4.
Supercoiled and relaxed DNA were resolved electrophoretically in the presence of 0.5 micrograms/ml ethidium bromide. Under these conditions the Gaussian distributions of topological isomers of both supercoiled and relaxed DNA migrated as discrete bands. The separation of these DNAs was optimized by varying the concentration of electrode buffer. Electrophoresis in the presence of 160 mM Tris-acetate, pH 8.3, 4 mM EDTA resulted in a 20-fold increase in the separation of relaxed and supercoiled DNA relative to electrophoresis in 40 mM Tris-acetate, pH 8.3, 1 mM EDTA.
超螺旋DNA和松弛型DNA在含有0.5微克/毫升溴化乙锭的条件下进行电泳分离。在这些条件下，超螺旋DNA和松弛型DNA拓扑异构体的高斯分布迁移为离散条带。通过改变电极缓冲液的浓度优化了这些DNA的分离效果。在160 mM Tris-乙酸盐（pH 8.3）、4 mM EDTA存在下进行电泳，相对于在40 mM Tris-乙酸盐（pH 8.3）、1 mM EDTA中进行电泳，松弛型DNA和超螺旋DNA的分离度提高了20倍。
