血管活性肠肽增强大鼠下丘脑原代细胞培养物中的多巴胺积累。
Vasoactive intestinal peptide enhances dopamine accumulation in primary cell culture of rat hypothalamus.
作者信息
Kadowaki K, Koike K, Hirota K, Ohmichi M, Kurachi H, Miyoke A, Tanizawa O
机构信息
Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
出版信息
Endocrinol Jpn. 1992 Apr;39(2):197-202. doi: 10.1507/endocrj1954.39.197.
The effect of vasoactive intestinal peptide (VIP) and PHI-27 on dopamine accumulation in cultured rat hypothalamic cells was investigated. VIP enhanced [3H]dopamine accumulation dose dependently. This effect was significant at 10(-8)-10(-5) M VIP with a concomitant increase in intracellular cyclic AMP (cAMP), and reached its plateau level at 10(-6) M VIP. VIP increased [3H]dopamine accumulation significantly within 15 min. PHI-27 and dibutyryl cAMP ((Bu)2-cAMP) also enhanced [3H]dopamine accumulation. These results suggest that VIP enhances dopamine accumulation in hypothalamic cells by increasing intracellular cAMP.
研究了血管活性肠肽（VIP）和PHI - 27对培养的大鼠下丘脑细胞中多巴胺积累的影响。VIP剂量依赖性地增强了[³H]多巴胺的积累。在10⁻⁸ - 10⁻⁵ M的VIP浓度下，这种作用显著，同时细胞内环磷酸腺苷（cAMP）增加，在10⁻⁶ M的VIP浓度下达到平台期。VIP在15分钟内显著增加了[³H]多巴胺的积累。PHI - 27和二丁酰环磷腺苷（（Bu）₂ - cAMP）也增强了[³H]多巴胺的积累。这些结果表明，VIP通过增加细胞内cAMP来增强下丘脑细胞中多巴胺的积累。
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