3',5'-环磷酸腺苷增强含有多巴胺能神经元的大鼠下丘脑细胞培养物中的多巴胺积累。

Adenosine 3',5'-cyclic monophosphate enhances dopamine accumulation in rat hypothalamic cell culture containing dopaminergic neurons.

作者信息

Kadowaki K, Hirota K, Koike K, Ohmichi M, Kiyama H, Miyake A, Tanizawa O

机构信息

Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.

出版信息

Neuroendocrinology. 1990 Sep;52(3):256-61. doi: 10.1159/000125595.

DOI:10.1159/000125595

PMID:2170853

Abstract

The regulation of dopamine accumulation in cultured rat hypothalamic cells by adenosine 3',5'-cyclic monophosphate (cAMP) was investigated in cultures of newborn rat hypothalamic cells. Both dibutyryl cAMP, (Bu)2-cAMP, and forskolin enhanced [3H]dopamine accumulation in a dose-dependent manner. cAMP also enhanced [3H]dopamine accumulation, but to a lesser degree. Neither n-butyrate nor adenosine alone enhanced [3H]dopamine accumulation. (Bu)2-cAMP had no effect on basal efflux of [3H]radioactivity. The effect of (Bu)2-cAMP appeared on day 5 of culture, reached a maximum on day 6, and then rapidly decreased. These results suggest that dopamine uptake by cultured rat hypothalamic cells is regulated by intracellular cAMP.

摘要

在新生大鼠下丘脑细胞培养物中，研究了3',5'-环磷酸腺苷（cAMP）对培养的大鼠下丘脑细胞中多巴胺积累的调节作用。二丁酰cAMP（(Bu)2-cAMP）和福斯可林均以剂量依赖的方式增强了[3H]多巴胺的积累。cAMP也增强了[3H]多巴胺的积累，但程度较小。单独的正丁酸盐或腺苷均未增强[3H]多巴胺的积累。(Bu)2-cAMP对[3H]放射性的基础流出没有影响。(Bu)2-cAMP的作用在培养的第5天出现，第6天达到最大值，然后迅速下降。这些结果表明，培养的大鼠下丘脑细胞对多巴胺的摄取受细胞内cAMP的调节。