Tepel M, Bauer S, Husseini S, Zidek W
Medizinische Universität-Poliklinik, Münster, Germany.
J Hypertens. 1992 Sep;10(9):991-6.
The role of intracellular Na+ concentration in the pathogenesis of essential hypertension is a point of considerable discussion.
Since the novel fluorescent dye technique offers the possibility of measuring cytosolic free Na+ concentration in intact living cells, the role of Na+ was reinvestigated in resting and stimulated human platelets.
Cytosolic free Na+ concentration was measured in intact blood platelets of 20 essential hypertensive patients and 21 age- and sex-matched normotensive control subjects using the fluorescent dye Na(+)-binding benzofuran isophthalate.
Cytosolic free Na+ concentration was significantly reduced in hypertensives compared with normotensives. Inhibition of Na+,K(+)-adenosine triphosphatase by ouabain elevated cytosolic free Na+ concentration in hypertensives and normotensives in a similar way. Addition of thrombin increased cytosolic free Na+ concentration both in hypertensives and normotensives.
Previous concepts concerning the role of Na+ in the pathogenesis of essential hypertension based upon measurements in destructed cells need to be reinvestigarted using new techniques in living cells.
细胞内钠离子浓度在原发性高血压发病机制中的作用是一个备受争议的问题。
由于新型荧光染料技术提供了测量完整活细胞中胞质游离钠离子浓度的可能性，因此在静息和受刺激的人类血小板中对钠离子的作用进行了重新研究。
使用荧光染料钠结合苯并呋喃间苯二甲酸，测量了20例原发性高血压患者和21例年龄及性别匹配的血压正常对照者完整血小板中的胞质游离钠离子浓度。
与血压正常者相比，高血压患者的胞质游离钠离子浓度显著降低。哇巴因对钠钾-腺苷三磷酸酶的抑制以类似方式升高了高血压患者和血压正常者的胞质游离钠离子浓度。添加凝血酶可增加高血压患者和血压正常者的胞质游离钠离子浓度。
以往基于对破坏细胞测量得出的关于钠离子在原发性高血压发病机制中作用的概念，需要利用活细胞新技术重新进行研究。
