Ring C J, Jones M D, Griffin B E
Department of Virology, Royal Postgraduate Medical School, London, U.K.
J Gen Virol. 1992 Oct;73 ( Pt 10):2715-9. doi: 10.1099/0022-1317-73-10-2715.
The Daudi strain of Epstein-Barr virus (EBV) possesses a genomic deletion, relative to the B95-8 EBV prototype, that removes the entire Epstein-Barr virus nuclear antigen 2 (EBNA2) open reading frame (ORF) and the sequences encoding the carboxy terminus of EBNA5. Immunoblot analysis carried out in this study indicates that two species of EBNA5 (31K and 37K) are expressed in Daudi cells. Nucleotide sequence analysis of Daudi cDNA clones has confirmed that, as a consequence of the genomic deletion, exons usually appearing further downstream in EBNA messages (exons U or HF) are spliced directly onto the truncated EBNA5 ORF. Furthermore, the use of alternative splicing suggests that the two EBNA5 species expressed in Daudi cells possess different carboxy termini.
相对于EBV原型B95 - 8，爱泼斯坦 - 巴尔病毒（EBV）的Daudi毒株存在基因组缺失，该缺失移除了整个爱泼斯坦 - 巴尔病毒核抗原2（EBNA2）开放阅读框（ORF）以及编码EBNA5羧基末端的序列。本研究进行的免疫印迹分析表明，Daudi细胞中表达了两种EBNA5（31K和37K）。对Daudi cDNA克隆的核苷酸序列分析证实，由于基因组缺失，通常在EBNA信息中更下游出现的外显子（外显子U或HF）直接剪接到截短的EBNA5 ORF上。此外，可变剪接的使用表明，Daudi细胞中表达的两种EBNA5具有不同的羧基末端。
