不同株爱泼斯坦-巴尔病毒中爱泼斯坦-巴尔病毒核抗原2基因的异质性。

Heterogeneity within the Epstein-Barr virus nuclear antigen 2 gene in different strains of Epstein-Barr virus.

作者信息

Aitken C, Sengupta S K, Aedes C, Moss D J, Sculley T B

机构信息

Queensland Institute of Medical Research, Herston, Brisbane, Australia.

出版信息

J Gen Virol. 1994 Jan;75 ( Pt 1):95-100. doi: 10.1099/0022-1317-75-1-95.

DOI:10.1099/0022-1317-75-1-95

PMID:8113744

Abstract

DNA isolated from biopsies of endemic Burkitt's lymphoma (BL) from New Guinea was analysed for the presence of Epstein-Barr virus (EBV) sequences using the polymerase chain reaction. Primers were designed to amplify sequences within the Epstein-Barr virus nuclear antigen (EBNA) 1 and 2 genes. These analyses detected the EBNA1 sequence in all the biopsies studied. Additional sets of primers directed against the EBNA2 gene were used in order to categorize the EBV strains as A-type or B-type (39% A-type; 50% B-type; 5% A- and B-type; 5% untypeable). These results indicated that DNA sequence heterogeneity within the EBNA2 gene region may exist in different strains of EBV. The extent of DNA sequence heterogeneity among different strains of EBV was determined by sequencing of a region within the EBNA2 gene in a number of different A-type and B-type strains of EBV originating from Africa or New Guinea. The results demonstrated DNA sequence heterogeneity within the EBNA2 gene in different strains of EBV. This heterogeneity was more extensive among A-type strains than B-type strains of EBV.

摘要

使用聚合酶链反应分析从新几内亚地方性伯基特淋巴瘤（BL）活检组织中分离出的DNA，以检测爱泼斯坦-巴尔病毒（EBV）序列的存在。设计引物以扩增爱泼斯坦-巴尔病毒核抗原（EBNA）1和2基因内的序列。这些分析在所有研究的活检组织中检测到了EBNA1序列。使用针对EBNA2基因的其他引物组，以便将EBV菌株分类为A型或B型（39%为A型；50%为B型；5%为A和B型；5%无法分型）。这些结果表明，EBNA2基因区域内的DNA序列异质性可能存在于不同的EBV菌株中。通过对来自非洲或新几内亚的多种不同A型和B型EBV菌株的EBNA2基因内一个区域进行测序，确定了不同EBV菌株之间DNA序列异质性的程度。结果表明，不同EBV菌株的EBNA2基因内存在DNA序列异质性。这种异质性在EBV的A型菌株中比B型菌株中更为广泛。