Nouel D, Costentin J, Lugrin D, Kitabgi P, Ple N, Davoust D
Unité de Neuropsychopharmacologie Expérimentale, U.R.A. 1170 du C.N.R.S., Saint Etienne du Rouvray, France.
J Neurochem. 1992 Nov;59(5):1933-6. doi: 10.1111/j.1471-4159.1992.tb11029.x.
Interactions between dopamine and neurotensin can occur at various levels of the dopaminergic pathways. By using different approaches in vitro, we investigated the proposed hypothesis that neurotensin might bind to dopamine in the synaptic cleft. Nuclear magnetic resonance spectra of neurotensin were not modified by the addition of dopamine, and no nuclear Overhauser effect was detected. Synaptosomal uptake of [3H]dopamine in the presence of neurotensin did not lead to any modifications of the kinetic constants of the uptake. Neurotensin binding was not modified by the addition of dopamine. These results did not confirm the suggestion that neurotensin can form a complex with dopamine.
多巴胺与神经降压素之间的相互作用可发生在多巴胺能通路的不同水平。通过在体外使用不同方法，我们对神经降压素可能在突触间隙与多巴胺结合这一假说进行了研究。添加多巴胺后，神经降压素的核磁共振谱未发生改变，且未检测到核Overhauser效应。在存在神经降压素的情况下，[3H]多巴胺的突触体摄取未导致摄取动力学常数的任何改变。添加多巴胺后，神经降压素的结合未发生改变。这些结果并未证实神经降压素可与多巴胺形成复合物这一推测。
