von Euler G, Fuxe K, Hansson T, Gustafsson J A
Department of Histology, Karolinska Institute, Stockholm, Sweden.
Toxicology. 1988 Apr;49(1):149-54. doi: 10.1016/0300-483x(88)90187-4.
The effects of treatment with toluene in vivo (80 ppm, 3 days, 6 h/day) and in vitro (19 mumol/ml) were analyzed on the binding characteristics of [3H]neurotensin in rat striatal membranes. Exposure to toluene in vivo did not produce any significant effects on the binding characteristics of [3H]neurotensin. However, the addition of toluene in vitro caused a trend for a decreased Bmax value and produced a significantly reduced KD value of [3H]neurotensin binding. The absence of effects at 80 ppm indicates that the neurotensin receptor is relatively insensitive to toluene exposure, in contrast to, e.g. the dopamine agonist binding sites. Furthermore, the toluene response of the neurotensin receptor, as seen after treatment in vitro, is different from the responses seen in many monoamine receptors, which show decreased affinities following toluene exposure. It is possible that toluene is mediating its effects on the neurotensin receptor by changing the lipid micro-environment in which the receptor is situated. Another explanation would be that toluene selectively acts on the monoamine receptors, e.g. the more sensitive dopamine receptors, which through receptor-receptor interactions would cause the response seen in the neurotensin receptor. However, it cannot be excluded that the suggested receptor-receptor interaction itself is affected by toluene.
分析了甲苯体内（80 ppm，3天，每天6小时）和体外（19 μmol/ml）处理对大鼠纹状体膜中[3H]神经降压素结合特性的影响。体内接触甲苯对[3H]神经降压素的结合特性未产生任何显著影响。然而，体外添加甲苯导致Bmax值有降低趋势，并使[3H]神经降压素结合的KD值显著降低。80 ppm时无影响表明神经降压素受体对甲苯暴露相对不敏感，这与例如多巴胺激动剂结合位点不同。此外，体外处理后观察到的神经降压素受体对甲苯的反应不同于许多单胺受体的反应，后者在甲苯暴露后亲和力降低。甲苯可能通过改变受体所处的脂质微环境来介导其对神经降压素受体的作用。另一种解释是甲苯选择性作用于单胺受体，例如更敏感的多巴胺受体，通过受体 - 受体相互作用导致在神经降压素受体中观察到的反应。然而，不能排除所提出的受体 - 受体相互作用本身受到甲苯影响的可能性。
