CRESCITELLI F
J Gen Physiol. 1956 Jan 20;39(3):423-35. doi: 10.1085/jgp.39.3.423.
From the retina of the land-locked population of the sea lamprey, Petromyzon marinus, a photolabile pigment was extracted which was identified spectrophotometrically as a member of the rhodopsin group of pigments. Using the absorption spectrum of a relatively pure solution and analysis by means of difference spectra, the peak of this pigment was placed at about 497 mmicro. The method of selective bleaching by light of different wave lengths revealed no significant amounts of any other pigment in the extracts. A similar pigment was also detected in retinal extracts of the Pacific Coast lamprey, Entospenus tridentatus. These results are significant for two reasons: (a) the lamprey is shown to be an example of an animal which spawns in fresh water but which is characterized by the presence of rhodopsin, rather than porphyropsin, in the retina; (b) the primitive phylogenetic position of the lamprey suggests that rhodopsin was the visual pigment of the original vertebrates.
从内陆海七鳃鳗（海七鳃鳗）的视网膜中提取出一种对光不稳定的色素，通过分光光度法鉴定其为视紫红质类色素的一员。利用相对纯溶液的吸收光谱并借助差示光谱进行分析，该色素的峰值位于约497微米处。通过不同波长光的选择性漂白方法表明，提取物中不存在任何其他大量色素。在太平洋沿岸七鳃鳗（三齿七鳃鳗）的视网膜提取物中也检测到了类似的色素。这些结果具有重要意义，原因有两点：（a）七鳃鳗被证明是一种在淡水中产卵但视网膜中存在视紫红质而非视紫蓝质的动物实例；（b）七鳃鳗在系统发育中的原始地位表明视紫红质是原始脊椎动物的视觉色素。
