Hurtado de Catalfo G E, Mandon E C, de Gómez Dumm I N
Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), CONICET-UNLP, Facultad de Ciencias Médicas, Argentina.
Lipids. 1992 Aug;27(8):593-8. doi: 10.1007/BF02536116.
The biosynthesis of arachidonic acid in Sertoli and Leydig cells isolated from the testes of mature rats has been investigated. Both types of cells incorporated [2-14C]eicosatrienoic acid from the incubation medium and transformed it into arachidonic acid. The administration of adrenocorticotropin (ACTH) to the rats decreased the delta 5 desaturating activity in the isolated testicular cells, while ACTH produced no changes in the uptake of the substrate. Similar results were obtained when ACTH was added to the incubation medium of cells isolated from non-hormone treated rats. The total fatty acid composition of the Sertoli cells isolated from ACTH-treated rats showed a significant increase in the relative percentage of 18:2n-6 and a decrease in the C20 and C22 polyenes. This may indicate that ACTH exerts an inhibitory effect on delta 6, delta 5 and delta 4 desaturase activities. Addition of corticosterone to the incubation medium also produced a significant decrease in arachidonic acid biosynthesis. Because ACTH is known to stimulate the release of corticosterone in vivo, both hormones may act cumulatively in the regulation of arachidonic acid metabolism in Sertoli and Leydig cells.
已对从成熟大鼠睾丸分离出的支持细胞和间质细胞中花生四烯酸的生物合成进行了研究。两种类型的细胞都从孵育培养基中摄取[2-¹⁴C]二十碳三烯酸并将其转化为花生四烯酸。给大鼠注射促肾上腺皮质激素（ACTH）会降低分离出的睾丸细胞中的Δ⁵去饱和活性，而ACTH对底物的摄取没有影响。当将ACTH添加到从未经激素处理的大鼠分离出的细胞的孵育培养基中时，也获得了类似的结果。从经ACTH处理的大鼠分离出的支持细胞的总脂肪酸组成显示，18:2n-6的相对百分比显著增加，C20和C22多烯减少。这可能表明ACTH对Δ⁶、Δ⁵和Δ⁴去饱和酶活性具有抑制作用。向孵育培养基中添加皮质酮也会使花生四烯酸的生物合成显著减少。由于已知ACTH在体内会刺激皮质酮的释放，这两种激素可能在支持细胞和间质细胞中花生四烯酸代谢的调节中起累积作用。
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