Forbes K J, Bruce K D, Ball A, Pennington T H
Department of Medical Microbiology, University of Aberdeen, Foresterhill, UK.
Mol Microbiol. 1992 Aug;6(15):2107-12. doi: 10.1111/j.1365-2958.1992.tb01384.x.
Length variations of Haemophilus influenzae outer membrane porin protein P2 were found at the DNA and protein levels, notably in non-capsulate strains. Protein length, measured by SDS-polyacrylamide gel electrophoresis, was found to correlate with the length of the gene, measured by polymerase chain reaction amplification, and ranged from 35-42 kDa and 970-1090 nucleotides, respectively. This represents a length variation of some 15%. The genetic location of these variations was studied by restriction enzyme mapping 10 of the non-capsulate strains revealing further polymorphisms at the DNA level. All 10 strains were distinct and differed from a type b strain. The conservation and assortment of the different restriction sites in the alleles is discussed in relation to the very great diversity previously described for this protein and of the whole genome itself in non-capsulate strains. The roles of selection, horizontal gene transfer, and transformation in generating this diversity are discussed.
在DNA和蛋白质水平上均发现了流感嗜血杆菌外膜孔蛋白P2的长度变异，尤其是在非荚膜菌株中。通过SDS-聚丙烯酰胺凝胶电泳测量的蛋白质长度与通过聚合酶链反应扩增测量的基因长度相关，分别为35 - 42 kDa和970 - 1090个核苷酸。这代表了约15%的长度变异。通过对10株非荚膜菌株进行限制性酶切图谱分析，研究了这些变异的基因定位，结果显示在DNA水平上存在进一步的多态性。所有10株菌株均不同，且与b型菌株不同。结合此前描述的该蛋白以及非荚膜菌株全基因组本身的高度多样性，讨论了等位基因中不同限制性位点的保守性和分类情况。还讨论了选择、水平基因转移和转化在产生这种多样性中的作用。
