Ostberg K L, Russell M W, Murphy T F
Department of Microbiology and Immunology, University at Buffalo, The State University of New York, Buffalo, New York, USA.
Mucosal Immunol. 2009 Jan;2(1):63-73. doi: 10.1038/mi.2008.70. Epub 2008 Oct 29.
Nontypeable Haemophilus influenzae (NTHI) is a significant cause of otitis media in children and exacerbations in patients with chronic obstructive pulmonary disease. Vaccine research for NTHI has focused on the outer membrane proteins (OMPs) of NTHI. The goal of this study was to evaluate mucosal and systemic immune responses to recombinant OMP P2 (rP2) of NTHI. Enzyme-linked immunosorbent assay (ELISA) demonstrated that both mucosal and systemic routes of immunization resulted in antibodies to rP2. Whole-cell ELISA and flow cytometry indicated that mucosal immunization induced antibodies to epitopes that are on the bacterial surface of the homologous strain as well as several heterologous strains. In contrast, systemic immunization induced antibodies to non-surface exposed epitopes. These data show for the first time that mucosal immunization of mice with rP2 induces antibodies that recognize surface exposed epitopes on multiple strains, indicating that P2 is a candidate for development of a mucosal vaccine for NTHI.
非分型流感嗜血杆菌（NTHI）是儿童中耳炎以及慢性阻塞性肺疾病患者病情加重的重要病因。针对NTHI的疫苗研究主要集中在NTHI的外膜蛋白（OMPs）上。本研究的目的是评估对NTHI重组OMP P2（rP2）的黏膜和全身免疫反应。酶联免疫吸附测定（ELISA）表明，黏膜免疫途径和全身免疫途径均可产生针对rP2的抗体。全细胞ELISA和流式细胞术表明，黏膜免疫诱导产生针对同源菌株以及几种异源菌株细菌表面表位的抗体。相比之下，全身免疫诱导产生针对非表面暴露表位的抗体。这些数据首次表明，用rP2对小鼠进行黏膜免疫可诱导产生识别多种菌株表面暴露表位的抗体，这表明P2是开发NTHI黏膜疫苗的一个候选对象。
