Herbst W M, Kummer W, Heym C
Department of Dermatology, University of Erlangen-Nürnberg.
Neuropeptides. 1992 Jun;22(2):89-92. doi: 10.1016/0143-4179(92)90060-a.
A monoclonal VIP-receptor antibody derived from a human adenocarcinoma cell line (HT 29) was used in combination with immunogold silver staining for the immunohistochemical demonstration of VIP-receptor (rec) immunoreactivity (IR) in paraffin-embedded human salivary glands (parotid, palatal, labial glands). VIP-rec-IR was localized to mucous endpieces of labial and--to a lesser extent--palatal glands, intercalated ducts of the parotid gland, and excretory ducts of all glands investigated. The findings correlate well with known effects of VIP on mucous release and electrolyte transport in salivary glands. Lack of VIP-rec-IR at serious acini may point to immunologically different receptor subtypes in these glands.
一种源自人腺癌细胞系（HT 29）的单克隆VIP受体抗体，与免疫金银染色法联合使用，用于免疫组织化学显示石蜡包埋的人唾液腺（腮腺、腭腺、唇腺）中的VIP受体（rec）免疫反应性（IR）。VIP-rec-IR定位于唇腺的黏液末端以及（程度较轻的）腭腺、腮腺的闰管和所有被研究腺体的排泄管。这些发现与VIP对唾液腺黏液分泌和电解质转运的已知作用密切相关。严重腺泡中缺乏VIP-rec-IR可能表明这些腺体中存在免疫上不同的受体亚型。
