Therkildsen M H, Mandel U, Thorn J, Christensen M, Dabelsteen E
Department of Pathology, Rigshospitalet, University of Copenhagen, Denmark.
J Histochem Cytochem. 1994 Sep;42(9):1251-9. doi: 10.1177/42.9.8064132.
Simple mucin-type carbohydrate antigens Tn, sialosyl-Tn and T are often markers of neoplastic transformation and have very limited expression in normal tissues. We performed an immunohistological study of simple mucin-type carbohydrate antigens, including H and A variants, with well-defined monoclonal antibodies (MAb) on frozen and paraffin-embedded normal salivary gland tissue from 22 parotid, 14 submandibular, six sublingual, and 13 labial glands to elucidate the simple mucin-type glycosylation pattern in relation to cyto- and histodifferentiation. The investigated carbohydrate structures were predominantly observed in the cell cytoplasm, most often in the supranuclear area, suggesting localization to the Golgi region, whereas ductal contents were unstained. Mucous acinar cells expressed Tn, sialosyl-Tn, and H and A antigens, regardless of glandular location. Serous acinar cells, on the other hand, expressed A, H, and inconstantly sialosyl-T, Tn, and sialosyl-Tn antigens in major salivary glands, whereas serous cells of minor (labial) salivary glands expressed H exclusively, Tn and sialosyl-T antigens inconstantly, but never sialosyl-Tn and A antigens. The difference may be related to a more simple cytodifferentiation of serous cells of minor (labial) salivary glands as compared with major salivary glands. Duct cells in major salivary glands expressed A, H, and inconstantly T, sialosyl-T, and Tn antigens, whereas minor (labial) salivary glands ducts exclusively expressed H, T and sialosyl-T antigens, differences that may be related to dissimilarities in the duct system. Myoepithelial cells and basal cells exclusively expressed T and sialosyl-T antigens, which may prove useful in studies of salivary gland tumors, since these cells are known to play a key role in the histological characteristics of some salivary gland tumors. The results indicate a similar glycosylation pattern in the different major salivary glands, whereas minor (labial) salivary gland differ slightly in serous and duct cells. The limited and exclusive intracellular expression of the immature Tn, sialosyl-Tn, and T antigens indicates that these structures may be of value as markers of salivary gland tumors.
简单黏蛋白型碳水化合物抗原Tn、唾液酸化Tn和T通常是肿瘤转化的标志物，在正常组织中表达非常有限。我们使用明确的单克隆抗体（MAb），对来自22个腮腺、14个下颌下腺、6个舌下腺和13个唇腺的冷冻及石蜡包埋的正常唾液腺组织进行了简单黏蛋白型碳水化合物抗原（包括H和A变体）的免疫组织学研究，以阐明与细胞和组织分化相关的简单黏蛋白型糖基化模式。所研究的碳水化合物结构主要在细胞质中观察到，最常见于核上区域，提示定位于高尔基体区域，而导管内容物未染色。黏液腺泡细胞表达Tn、唾液酸化Tn以及H和A抗原，与腺体位置无关。另一方面，浆液性腺泡细胞在大唾液腺中表达A、H，偶尔表达唾液酸化T、Tn和唾液酸化Tn抗原，而小（唇）唾液腺的浆液细胞仅表达H，偶尔表达Tn和唾液酸化T抗原，但从不表达唾液酸化Tn和A抗原。这种差异可能与小（唇）唾液腺的浆液细胞与大唾液腺相比细胞分化更简单有关。大唾液腺中的导管细胞表达A、H，偶尔表达T、唾液酸化T和Tn抗原，而小（唇）唾液腺导管仅表达H、T和唾液酸化T抗原，这些差异可能与导管系统的不同有关。肌上皮细胞和基底细胞仅表达T和唾液酸化T抗原，这在唾液腺肿瘤研究中可能有用，因为已知这些细胞在某些唾液腺肿瘤的组织学特征中起关键作用。结果表明不同大唾液腺中存在相似的糖基化模式，而小（唇）唾液腺在浆液细胞和导管细胞中略有不同。未成熟的Tn、唾液酸化Tn和T抗原有限且仅在细胞内表达，表明这些结构可能作为唾液腺肿瘤的标志物具有价值。
