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粘质沙雷氏菌核酸酶活性需要二硫键。

Disulfide bonds are required for Serratia marcescens nuclease activity.

作者信息

Ball T K, Suh Y, Benedik M J

机构信息

Department of Biochemical and Biophysical Sciences, University of Houston, TX 77204-5934.

出版信息

Nucleic Acids Res. 1992 Oct 11;20(19):4971-4. doi: 10.1093/nar/20.19.4971.

DOI:10.1093/nar/20.19.4971
PMID:1329033
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC334271/
Abstract

The role of the two disulfide bonds found in the Serratia marcescens nuclease were tested by site directed mutagenesis and were found essential for nuclease activity, although slight residual activity remained. The requirement for disulfide bond formation may play a role in preventing the lethal action of nuclease while in the bacterial cytoplasm.

摘要

通过定点诱变对粘质沙雷氏菌核酸酶中发现的两个二硫键的作用进行了测试，结果发现它们对核酸酶活性至关重要，尽管仍有轻微的残余活性。二硫键形成的必要性可能在防止核酸酶在细菌细胞质中产生致死作用方面发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82d6/334271/135d8e588b1c/nar00230-0019-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82d6/334271/135d8e588b1c/nar00230-0019-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82d6/334271/135d8e588b1c/nar00230-0019-a.jpg

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