核酸酶对粘质沙雷氏菌转化效率的影响。
The effect of nuclease on transformation efficiency in Serratia marcescens.
作者信息
Palomar J, Guasch J F, Regué M, Viñas M
机构信息
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Barcelona, Spain.
出版信息
FEMS Microbiol Lett. 1990 Jun 1;57(3):255-8. doi: 10.1016/0378-1097(90)90076-3.
No differences in the efficiency of transformation were observed from both plasmid and chromosomal DNA in Serratia marcescens 2170 and an extracellular nuclease defective isogenic strain. The efficiency of transformation was the same for Escherichia coli 5K and E. coli containing a recombinant plasmid conferring the ability to synthesize a S. marcescens nuclease. From these results we conclude that the extracellular nuclease of S. marcescens 2170 is not the main cause of the low efficiency of transformation observed in this bacterium.
在粘质沙雷氏菌2170及其细胞外核酸酶缺陷同基因菌株中，未观察到质粒DNA和染色体DNA转化效率的差异。大肠杆菌5K和含有赋予合成粘质沙雷氏菌核酸酶能力的重组质粒的大肠杆菌的转化效率相同。从这些结果我们得出结论，粘质沙雷氏菌2170的细胞外核酸酶不是该细菌中观察到的低转化效率的主要原因。
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