de Blic J, Peuchmaur M, Carnot F, Danel C, Deruesne M, Reynaud P, Scheinmann P, Brousse N
Service de Pneumologie Pédiatrique and d'Anatomie Pathologique, Hôpital Necker-Enfants Malades, Paris, France.
Transplantation. 1992 Oct;54(4):639-44. doi: 10.1097/00007890-199210000-00014.
The number, distribution, and phenotype of mononuclear cells infiltrating the allograft lung transplant were determined immunohistochemically with monoclonal antibodies directed against cellular antigens (CD3, CD4, CD8, CD22, CD25, CD16, CD56, CD68, HLA-DR) on frozen sections of transbronchial biopsies. Seventy-two transbronchial biopsies from 21 patients undergoing lung or heart-lung transplantation were evaluated histologically and immunohistologically in a prospective study. Four major results were obtained in the graft lung parenchyma: (1) whatever the histological grading of rejection, T lymphocytes expressing CD3 were present and in a significantly higher number than in control subjects (P < 0.0005); (2) there was a positive correlation between histological rejection and the number of CD3+, CD8+, CD25+, CD16+ cells (P < 0.01); (3) the CD4/CD8 ratio was inverted (0.52 +/- 0.04), with no correlation with the histological rejection; and (4) the number and location of CD3+, CD25+ cells did not correlate with CMV identification in bronchoalveolar lavage. Immunohistochemical criteria could be used for diagnosis of rejection in the management of heart-lung transplantation.
采用针对细胞抗原（CD3、CD4、CD8、CD22、CD25、CD16、CD56、CD68、HLA-DR）的单克隆抗体，对经支气管活检的冷冻切片进行免疫组织化学检测，以确定同种异体肺移植中浸润的单核细胞数量、分布及表型。在一项前瞻性研究中，对21例接受肺移植或心肺联合移植患者的72份经支气管活检标本进行了组织学和免疫组织化学评估。在移植肺实质中获得了四项主要结果：（1）无论排斥反应的组织学分级如何，表达CD3的T淋巴细胞均存在，且数量显著高于对照组（P<0.0005）；（2）组织学排斥反应与CD3⁺、CD8⁺、CD25⁺、CD16⁺细胞数量呈正相关（P<0.01）；（3）CD4/CD8比值倒置（0.52±0.04），与组织学排斥反应无关；（4）CD3⁺、CD25⁺细胞的数量和位置与支气管肺泡灌洗中巨细胞病毒的鉴定无关。免疫组织化学标准可用于心肺移植管理中的排斥反应诊断。
