Chen H L, Lung M M, Sham J S, Choy D T, Griffin B E, Ng M H
Department of Microbiology, University of Hong Kong.
Virology. 1992 Nov;191(1):193-201. doi: 10.1016/0042-6822(92)90181-n.
Analysis by Northern blotting and sequencing of cDNA clones from a transcription library of a tumor biopsy from a nasopharyngeal carcinoma (NPC) patient showed that the BamHI-A region of the Epstein-Barr virus genome is abundantly and regularly transcribed in tumor tissues from NPC patients. The transcription occurred in a rightward direction terminating between coordinates 160,965 and 160,995, where two polyadenylation sites are located. Rightward transcription of this region also occurred in B lymphoid cells harboring the viral genome, albeit at a lower level than in the tumor tissues. Differential splicing yields a family of related transcripts displaying at least four splicing patterns. Different promoters may be utilized, further contributing to the diversity of this family of transcripts. A 2.8-kb unspliced transcript present in B95-8 cells was probably initiated from a TATA box located in position 158,204, while the other transcripts may utilize other promoters localized to other regions. All the transcripts encompass a putative open reading frame, BARFO, which is predicted to encode a basic protein of about 20 kDa. It shares 40% colinear amino acid sequence homology with the DNA binding region of a transcription factor, ICP4, specified by herpes simplex virus.
通过对一名鼻咽癌（NPC）患者肿瘤活检转录文库的cDNA克隆进行Northern印迹分析和测序，结果显示，爱泼斯坦-巴尔病毒基因组的BamHI-A区域在NPC患者的肿瘤组织中大量且有规律地转录。转录方向为向右，终止于坐标160,965和160,995之间，这两个位置存在两个聚腺苷酸化位点。该区域在携带病毒基因组的B淋巴细胞中也发生向右转录，尽管其水平低于肿瘤组织。差异剪接产生了一系列相关转录本，显示出至少四种剪接模式。可能使用了不同的启动子，这进一步增加了该转录本家族的多样性。B95-8细胞中存在的一个2.8kb未剪接转录本可能起始于位于位置158,204的一个TATA框，而其他转录本可能使用位于其他区域的其他启动子。所有转录本都包含一个推定的开放阅读框BARFO，预计它编码一种约20kDa的碱性蛋白。它与单纯疱疹病毒指定的转录因子ICP4的DNA结合区域具有40%的共线性氨基酸序列同源性。
