Sompayrac L, Danna K J
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80302.
Virology. 1992 Nov;191(1):439-42. doi: 10.1016/0042-6822(92)90206-5.
An SV40 mutant, T147D, encodes only the amino-terminal 147 amino acids of large T antigen and does not make small t antigen. We show here that a retrovirus which expresses this mutant T antigen transforms rat REF52 cells as efficiently as a retrovirus that expresses both the wild-type large and small T antigens. This cell line had previously been refractory to transformation by mutants that make short, amino-terminal fragments of T antigen.
一种SV40突变体T147D仅编码大T抗原的氨基末端147个氨基酸，不产生小t抗原。我们在此表明，表达这种突变T抗原的逆转录病毒转化大鼠REF52细胞的效率与表达野生型大T抗原和小t抗原的逆转录病毒相同。该细胞系以前对产生T抗原氨基末端短片段的突变体转化具有抗性。
