Ishii K, Yamato K, Kubonishi I, Taguchi H, Ohtsuki Y, Miyoshi I
Department of Medicine, Kochi Medical School, Japan.
Am J Hematol. 1992 Nov;41(3):218-24. doi: 10.1002/ajh.2830410314.
A 72-year-old man presented with a left testicular tumor and underwent orchiectomy. The tumor was massively infiltrated with myeloma cells bearing monoclonal cytoplasmic IgD lambda. Three months after orchiectomy, he developed huge abdominal masses and subsequently ascites containing numerous myeloma cells. An IgD-secreting myeloma cell line, designated delta-47, was established from the ascites. This cell line expressed CD4 and CD38, but lacked Fc and complement receptors, surface immunoglobulin, CD19, HLA-DR, and PCA-1. CD30 was detected on the cultured cells but not on the ascites tumor cells. Delta-47 cells secreted the same immunoglobulin (IgD lambda) as was found in the patient's serum. The light chain had a molecular weight of 35 kD which was larger than that of the normal light chain. Chromosome analysis of delta-47 revealed an aneuploid karyotype with complex abnormalities including 1q+, 2p+, and 14q+. To our knowledge, this is the only IgD-secreting myeloma cell line and would provide a useful tool for the study of IgD production and IgD myeloma.
一名72岁男性因左侧睾丸肿瘤就诊并接受了睾丸切除术。肿瘤大量浸润了携带单克隆胞质IgD λ的骨髓瘤细胞。睾丸切除术后三个月，他出现了巨大的腹部肿块，随后出现含有大量骨髓瘤细胞的腹水。从腹水中建立了一株分泌IgD的骨髓瘤细胞系，命名为delta-47。该细胞系表达CD4和CD38，但缺乏Fc和补体受体、表面免疫球蛋白、CD19、HLA-DR和PCA-1。在培养的细胞上检测到CD30，但在腹水肿瘤细胞上未检测到。Delta-47细胞分泌的免疫球蛋白（IgD λ）与患者血清中发现的相同。轻链的分子量为35 kD，大于正常轻链。Delta-47的染色体分析显示为非整倍体核型，伴有复杂异常，包括1q+、2p+和14q+。据我们所知，这是唯一一株分泌IgD的骨髓瘤细胞系，将为研究IgD产生和IgD骨髓瘤提供一个有用的工具。
