Lai M C, Beilharz M W, Scalzo A A, Garrett K L, Cannon J F, Boyer S J, Swaminathan N
Department of Microbiology, University of Western Australia, Nedlands.
Antiviral Res. 1992 May;18(1):65-76. doi: 10.1016/0166-3542(92)90006-q.
Site-directed in vitro mutagenesis followed by in vitro transcription and translation has been used to study structure/function relationships for murine interferon-alpha 1 (MuIFN-alpha 1). The mature form of the MuIFN-alpha 1 protein was expressed as well as analogue forms with amino acid substitutions at positions 33, 71, 72, 123 and 133. These positions were chosen on the basis of known human interferon-alpha structure/function relationships. Biological assays for antiviral activity on murine cells and natural killer cell activation have been performed for each of the proteins produced. The data obtained have been interpreted in the light of previous human and murine interferon-alpha structure/function work and the recently published three-dimensional structure of murine type I interferon.
通过定点体外诱变，随后进行体外转录和翻译，已用于研究小鼠α-干扰素1（MuIFN-α1）的结构/功能关系。表达了MuIFN-α1蛋白的成熟形式以及在第33、71、72、123和133位氨基酸有取代的类似形式。这些位置是根据已知的人α-干扰素结构/功能关系选定的。已对所产生的每种蛋白质进行了针对小鼠细胞抗病毒活性和自然杀伤细胞激活的生物学测定。已根据先前关于人和小鼠α-干扰素结构/功能的研究工作以及最近发表的小鼠I型干扰素的三维结构对所获得的数据进行了解释。
