Swaminathan N, Lai C M, Beilharz M W, Boyer S J, Klinken S P
Department of Microbiology, University of Western Australia, Nedlands.
Antiviral Res. 1992 Aug;19(2):149-59. doi: 10.1016/0166-3542(92)90074-f.
The mature forms of two recombinant murine interferons alpha, alpha 1 and alpha 4, have been expressed in vitro using an established transcription and translation system. The relative specific antiviral activity, antiproliferative activity and the natural killer cell stimulating activity of both subtypes were compared in vitro. While the antiviral and natural killer cell stimulating activities of the 2 subtypes were similar, the relative antiproliferative activities varied markedly. On the basis of equal molar inputs, MuIFN-alpha 1 had less than 8% of the antiproliferative activity of MuIFN-alpha 4. This data shows that a large functional difference exists between these two subtypes which are known to be expressed at different levels in mouse L-cells in vitro.
利用已建立的转录和翻译系统，在体外表达了两种重组鼠干扰素α的成熟形式，即α1和α4。在体外比较了这两种亚型的相对特异性抗病毒活性、抗增殖活性和自然杀伤细胞刺激活性。虽然这两种亚型的抗病毒和自然杀伤细胞刺激活性相似，但相对抗增殖活性却有显著差异。基于等摩尔输入量，MuIFN-α1的抗增殖活性不到MuIFN-α4的8%。该数据表明，这两种亚型之间存在很大的功能差异，已知它们在体外小鼠L细胞中的表达水平不同。
