Hirai H, Natori S, Sekimizu K
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Arch Biochem Biophys. 1992 Nov 1;298(2):458-63. doi: 10.1016/0003-9861(92)90435-y.
We examined the effects of phospholipids on transcription and replication in vitro in the presence of histones. Phosphatidylglycerol and cardiolipin were shown to reverse the inhibitory effects of histones in both random RNA synthesis with purified RNA polymerase II and accurate transcription initiated from the adenovirus 2 major late promoter in a nuclear extract. Phosphatidylserine, phosphatidic acid, and phosphatidylcholine did not activate RNA synthesis although they bound as strongly as phosphatidylglycerol and cardiolipin to histones. Phosphatidylglycerol and cardiolipin also reversed the in vitro inhibition of SV40 DNA replication by histones. Unsaturation of the fatty acid residues was shown to be necessary for the restoration of transcription and replication by phosphatidylglycerol.
我们研究了在组蛋白存在的情况下磷脂对体外转录和复制的影响。结果表明，磷脂酰甘油和心磷脂在使用纯化的RNA聚合酶II进行的随机RNA合成以及核提取物中腺病毒2主要晚期启动子起始的精确转录过程中，均可逆转组蛋白的抑制作用。磷脂酰丝氨酸、磷脂酸和磷脂酰胆碱虽然与磷脂酰甘油和心磷脂一样强烈地结合组蛋白，但并未激活RNA合成。磷脂酰甘油和心磷脂也可逆转组蛋白对体外SV40 DNA复制的抑制作用。结果显示，脂肪酸残基的不饱和对于磷脂酰甘油恢复转录和复制是必要的。
