Keller L H, Benson C E, Davison S, Eckroade R J
School of Veterinary Medicine, University of Pennsylvania, New Bolton Center, Kennett Square 19348.
Avian Dis. 1992 Jul-Sep;36(3):575-81.
Restriction endonuclease fingerprints of infectious laryngotracheitis virus (ILTV) DNA from 13 Pennsylvania field isolates, embryo-propagated and tissue-culture-propagated vaccine strains, and three reference strains were compared. These comparisons were made to evaluate the possible contribution of mutation of ILTV vaccine strains to recent outbreaks of infectious laryngotracheitis (ILT) in Pennsylvania. Six different restriction enzymes were used to generate the fingerprints. Differences in DNA banding patterns were revealed between the currently used ILTV vaccine strains and six of the 13 field isolates. Even greater DNA banding pattern differences were found between the older ILTV reference strains and the vaccine strains. The ILTV DNA fingerprints generated in the present study suggest that at least five different strains of ILTV have contributed to the outbreaks of ILT that have occurred since 1987 in Pennsylvania.
对来自宾夕法尼亚州的13株野外分离株、鸡胚传代和组织培养传代的传染性喉气管炎病毒（ILTV）疫苗株以及三株参考株的DNA进行了限制性内切酶指纹图谱比较。进行这些比较是为了评估ILTV疫苗株的突变对宾夕法尼亚州近期传染性喉气管炎（ILT）疫情爆发可能产生的影响。使用六种不同的限制性内切酶来生成指纹图谱。目前使用的ILTV疫苗株与13株野外分离株中的6株之间的DNA条带模式存在差异。在较老的ILTV参考株与疫苗株之间发现了更大的DNA条带模式差异。本研究中生成的ILTV DNA指纹图谱表明，自1987年以来，至少五种不同的ILTV毒株导致了宾夕法尼亚州ILT疫情的爆发。
