Andreasen J R, Glisson J R, Villegas P
Department of Avian Medicine, College of Veterinary Medicine, University of Georgia, Athens 30605.
Avian Dis. 1990 Jul-Sep;34(3):646-56.
Seven restriction endonucleases (REs) were used to cleave the DNA from seven vaccine strains of infectious laryngotracheitis (ILT) virus and from six Georgia field isolates of ILT virus. After electrophoresis of the resulting RE fragments, the patterns were compared in order to differentiate strains of ILT virus. The six chicken-embryo-origin (CEO) vaccines were identical with each RE, but the tissue-culture-origin (TCO) vaccine strain differed from the CEO vaccines using five of the REs. Four of the six field isolates were identical by each RE, but two field isolates differed from each other and from the four identical field isolates on the basis of patterns produced by some but not all of the REs. The four identical field isolates could not be differentiated from the CEO vaccine strains by any RE, but the other two field isolates were not identical to either strain of vaccine virus. This work demonstrates that differentiable strains of ILT virus exist in the United States and that viruses other than vaccine viruses are involved in field outbreaks of ILT.
使用七种限制性内切酶（REs）切割来自七种传染性喉气管炎（ILT）病毒疫苗株以及六种佐治亚州ILT病毒野外分离株的DNA。对所得的RE片段进行电泳后，比较其图谱以区分ILT病毒株。六种鸡胚源（CEO）疫苗在用每种RE切割时图谱相同，但组织培养源（TCO）疫苗株在用五种RE切割时与CEO疫苗不同。六种野外分离株中的四种在用每种RE切割时图谱相同，但另外两种野外分离株彼此不同，并且与四种图谱相同的野外分离株相比，在部分而非全部RE产生的图谱基础上也有所不同。四种图谱相同的野外分离株无法通过任何一种RE与CEO疫苗株区分开来，但另外两种野外分离株与任何一种疫苗病毒株都不相同。这项工作表明美国存在可区分的ILT病毒株，并且野外ILT疫情涉及疫苗病毒以外的病毒。
