Ishii N, Wadsworth W G, Stern B D, Culotti J G, Hedgecock E M
Department of Cell and Developmental Biology, Roche Institute of Molecular Biology, Nutley, New Jersey 07110.
Neuron. 1992 Nov;9(5):873-81. doi: 10.1016/0896-6273(92)90240-e.
The unc-6 gene is required for the guidance of pioneer axons and migrating cells along the body wall in C. elegans. In mutants, dorsal and ventral migrations are disrupted, but longitudinal movements are largely unaffected. The gene was tagged for molecular cloning by two independent transposon insertions. Based on genomic and cDNA sequencing, the gene encodes a novel laminin-related protein, UNC-6 (591 amino acids). The N-terminus is homologous to the N-termini (i.e., domains V1, V-1, V-2, and V-3) of laminin subunits, while the C-terminus is a unique domain. We propose that UNC-6 is a component of an extracellular matrix cue that guides dorsoventral migrations on the epidermis.
在秀丽隐杆线虫中，unc-6基因对于先驱轴突以及沿体壁迁移的细胞的导向是必需的。在突变体中，背侧和腹侧迁移受到破坏，但纵向运动基本不受影响。该基因通过两个独立的转座子插入进行分子克隆标记。基于基因组和cDNA测序，该基因编码一种新型的层粘连蛋白相关蛋白UNC-6（591个氨基酸）。其N端与层粘连蛋白亚基的N端（即结构域V1、V-1、V-2和V-3）同源，而C端是一个独特的结构域。我们认为UNC-6是一种细胞外基质信号的组成部分，可引导表皮上的背腹迁移。
