神经元5-羟色胺受体拮抗剂对豚鼠肠兴奋性反射中非胆碱能神经节传递的影响。
The influence of neuronal 5-hydroxytryptamine receptor antagonists on non-cholinergic ganglionic transmission in the guinea-pig enteric excitatory reflex.
作者信息
Tonini M, Coccini T, Onori L, Candura S M, Rizzi C A, Manzo L
机构信息
Department of Internal Medicine and Therapeutics, University of Pavia, Italy.
出版信息
Br J Pharmacol. 1992 Sep;107(1):5-7. doi: 10.1111/j.1476-5381.1992.tb14455.x.
A partitioned bath made it possible to separate the site of recording of the ascending excitatory reflex of the ileal circular muscle (oral compartment) from the site of reflex induction (caudal compartment), evoked by inflating an intraluminal balloon. In the caudal compartment, blockade of cholinergic ganglionic transmission by hexamethonium (100 microM) and hyoscine (0.3 microM) caused an approximately 65% reduction in the amplitude of reflex contractions, suggesting that the remaining response was mediated by non-cholinergic transmission near the distension site. This non-cholinergic component of ganglionic transmission was insensitive to the action of methiothepin (1 microM), ondansetron (1 microM), tropisetron (1.5 microM), DAU 6285 (1 microM) and renzapride (1 microM), agents that antagonize the action of 5-hydroxytryptamine (5-HT) at neural 5-HT1-like, 5-HT3, 5-HT4 and putative 5-HT1P receptors. These findings suggest that the neural pathways subserving non-cholinergic ganglionic transmission in the ascending excitatory reflex in the guinea-pig ileum do not involve 5-HT as neurotransmitter.
一个分隔式浴槽使得能够将回肠环形肌升支兴奋性反射的记录部位（口腔隔室）与反射诱导部位（尾侧隔室）分开，反射由向腔内气囊充气诱发。在尾侧隔室中，六甲铵（100微摩尔）和东莨菪碱（0.3微摩尔）对胆碱能神经节传递的阻断导致反射收缩幅度降低约65%，这表明剩余反应是由扩张部位附近的非胆碱能传递介导的。神经节传递的这种非胆碱能成分对甲硫噻平（1微摩尔）、昂丹司琼（1微摩尔）、托烷司琼（1.5微摩尔）、DAU 6285（1微摩尔）和瑞扎曲普坦（1微摩尔）的作用不敏感，这些药物可拮抗5-羟色胺（5-HT）在神经5-HT1样、5-HT3、5-HT4和假定的5-HT1P受体上的作用。这些发现表明，豚鼠回肠升支兴奋性反射中支持非胆碱能神经节传递的神经通路不涉及5-HT作为神经递质。
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