乳清酸、2-硫代乳清酸和异乳清酸对肝癌细胞核苷酸代谢及核酸合成的抑制作用。

Inhibitory action of orotate, 2-thioorotate and isoorotate on nucleotide metabolism and nucleic acid synthesis in hepatoma cells.

Lea M A, Luke A, Assad A, Patel M, Reddy P A

Department of Biochemistry and Molecular Biology, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark 07103.

Int J Biochem. 1992 Sep;24(9):1453-9. doi: 10.1016/0020-711x(92)90071-8.

Abstract

The specificity of the action of orotate on hepatoma cells was investigated. 2. Orotic acid and its methyl ester had similar inhibitory effects on the incorporation of [3H]thymidine into DNA of hepatoma cells. 3. In contrast to previous studies in vivo, incubation of rat kidney cells with orotate caused an increase in the ratio of UTP/ATP concentrations that was similar to effects on hepatic cells. 4. Inhibitory effects of 2-thioorotate and isoorotate on metabolism were found to be less selective and required higher concentrations than with orotate.

摘要

研究了乳清酸对肝癌细胞作用的特异性。2. 乳清酸及其甲酯对[3H]胸腺嘧啶核苷掺入肝癌细胞DNA具有相似的抑制作用。3. 与先前的体内研究不同，用乳清酸孵育大鼠肾细胞会导致UTP/ATP浓度比值增加，这与对肝细胞的作用相似。4. 发现2-硫代乳清酸和异乳清酸对代谢的抑制作用选择性较低，且与乳清酸相比需要更高的浓度。