Yamoto M, Shima K, Nakano R
Department of Obstetrics and Gynecology, Wakayama Medical College, Japan.
Horm Res. 1992;37 Suppl 1:5-11. doi: 10.1159/000182335.
To study changes in the binding of gonadotropins to human follicles and corpora lutea, we have examined the binding of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in the human ovary throughout the menstrual cycle, by both quantitative binding assays and surface binding autoradiography. The specific high-affinity low-capacity receptors for hLH were demonstrated in both human follicles and corpora lutea. Binding of 125I-hFSH was identified in the granulosa cells, but not in the thecal cells of the preantral and antral follicles at various stages of follicular development. The binding of 125I-hLH to the thecal cells increased during follicular development, and a dramatic increase was preferentially observed in the granulosa cells of the preovulatory follicles. In the corpora lutea, the binding of 125I-hLH increased from the early luteal phase to the midluteal phase and decreased towards the late luteal phase. The results of the present study suggest that changes in the binding of gonadotropins in the ovarian target cells during the menstrual cycle might play an important role in the regulation of follicular and luteal function.
为研究促性腺激素与人卵泡及黄体结合的变化，我们通过定量结合测定和表面结合放射自显影技术，对整个月经周期人卵巢中促卵泡激素（FSH）和促黄体生成素（LH）的结合情况进行了检测。在人卵泡和黄体中均证实存在hLH的特异性高亲和力低容量受体。在卵泡发育各阶段的窦前卵泡和窦状卵泡的颗粒细胞中可检测到125I-hFSH的结合，但在其膜细胞中未检测到。在卵泡发育过程中，125I-hLH与膜细胞的结合增加，且在排卵前卵泡的颗粒细胞中优先观察到显著增加。在黄体中，125I-hLH的结合从黄体早期到中期增加，而在黄体晚期减少。本研究结果表明，月经周期中卵巢靶细胞促性腺激素结合的变化可能在卵泡和黄体功能的调节中起重要作用。
