Eis-Hübinger A M, Friedrich A, Schneweis K E
Institute of Medical Microbiology and Immunology, University of Bonn, FRG.
Intervirology. 1992;34(1):13-22. doi: 10.1159/000150258.
Mouse hybridoma clones were examined for their ability to support replication of herpes simplex virus (HSV). Infection of hybridoma clone 1 cells producing an antibody not specific for HSV resulted in a persistent infection with a continuous production of infectious virus, whereas infection of the parental myeloma cells X63-Ag8.653 led to an abundant virus production and extinction of the culture. In contrast, infection of hybridoma cells producing HSV-specific antibodies was restricted to a few weeks. Infectious virus was isolated for a maximum of 10 days and, afterwards, viral antigens were detected by immunofluorescence for a maximum of 18 days. The neutralizing capacity of the antibodies was not essential since the pattern of infection in clone III E8 cells, producing a non-neutralizing antibody, did not differ from that observed in clones 2c and VI A6, which produced highly and weakly neutralizing antibodies, respectively. After loss of viral antigen, HSV DNA was no longer detected by Southern blot hybridization in hybridoma clone 2c cells. Since no difference other than the specificity of the produced antibodies is suspected between the hybridoma clones, the results suggest that the presence of HSV-specific antibodies in the B-lymphoid cell cultures is responsible for virus elimination from the cells.
对小鼠杂交瘤克隆进行检测，以评估其支持单纯疱疹病毒（HSV）复制的能力。用不针对HSV的抗体感染产生杂交瘤克隆1细胞，导致持续感染并持续产生感染性病毒，而亲代骨髓瘤细胞X63-Ag8.653的感染则导致大量病毒产生并使培养物灭绝。相比之下，用产生HSV特异性抗体的杂交瘤细胞进行感染的时间限制在几周内。最多10天可分离到感染性病毒，之后，通过免疫荧光检测病毒抗原的时间最长为18天。抗体的中和能力并非至关重要，因为产生非中和抗体的克隆III E8细胞中的感染模式与分别产生高度中和抗体和弱中和抗体的克隆2c和VI A6中的感染模式并无差异。在病毒抗原消失后，通过Southern印迹杂交在杂交瘤克隆2c细胞中不再检测到HSV DNA。由于除所产生抗体的特异性外，怀疑杂交瘤克隆之间没有其他差异，因此结果表明B淋巴细胞培养物中存在HSV特异性抗体是导致病毒从细胞中清除的原因。
