Pereira L, Klassen T, Baringer J R
Infect Immun. 1980 Aug;29(2):724-32. doi: 10.1128/iai.29.2.724-732.1980.
Hybridoma cells produced by fusing mouse myeloma cells with spleen cells from mice primed with herpes simplex virus (HSV) type 1 yielded five clones producing neutralizing antibody against homologous virus. Two clones, HCl and HC2, produced antibody capable of precipitating glycoprotein C and its precursor, whereas three clones, HD1, HD2, and HD3, produced antibody capable of precipitating glycoprotein D and its precursor. Antibody produced by the HC1 and HC2 clones neutralized HSV type 1 but not HSV type 2 or HSV type 1 strain MP, which is known to lack glycoprotein C. Antibody produced by the HD1 and HD2 clones neutralized both HSV type 1 and HSV type 2, whereas antibody produced by the HD3 clone neutralized HSV type 1 but not HSV type 2. The two clones which produced antibody to glycoprotein C and the two clones which produced type-common antibody to glycoprotein D were independently derived and not clonally related inasmuch as the antibody in each pair belonged to a different subclass of immunoglobulin.
将小鼠骨髓瘤细胞与用1型单纯疱疹病毒（HSV）免疫的小鼠脾细胞融合产生的杂交瘤细胞，产生了五个能产生针对同源病毒的中和抗体的克隆。两个克隆，即HCl和HC2，产生的抗体能够沉淀糖蛋白C及其前体，而三个克隆，即HD1、HD2和HD3，产生的抗体能够沉淀糖蛋白D及其前体。由HC1和HC2克隆产生的抗体可中和1型HSV，但不能中和2型HSV或已知缺乏糖蛋白C的1型HSV毒株MP。由HD1和HD2克隆产生的抗体可中和1型和2型HSV，而由HD3克隆产生 的抗体可中和1型HSV，但不能中和2型HSV。产生针对糖蛋白C的抗体的两个克隆和产生针对糖蛋白D的共同型抗体的两个克隆是独立衍生的，且不存在克隆相关性，因为每对抗体属于免疫球蛋白的不同亚类。
