雌激素作用下家兔子宫中酸性复合糖的代谢率

Metabolic rate of acidic complex saccharides in rabbit uterus under estrogenic condition.

作者信息

Endo M, Yosizawa Z

出版信息

J Biochem. 1976 Jan;79(1):1-4. doi: 10.1093/oxfordjournals.jbchem.a131035.

DOI:10.1093/oxfordjournals.jbchem.a131035

Abstract

Uterine slices obtained from estrogen-treated rabbits were incubated in vitro with N-acetyl-D-[1-3H]glucosamine together with D-[U-14C]glucose. The isotope-labelled acidic complex saccharides were then isolated by pronase digestion, Dowex 1 column chromatography and preparative electrophoresis on cellulose acetate membrane, in succession. In this way, individual acidic complex saccharides (hyaluronic acid, heparan sulfate, chondroitin sulfate A, chondroitin sulfate C, dermatan sulfate, sulfated glycopeptide, and sialoglycopeptide) were separated into 2-5 subfractions. The specific radioactivity of hexosamine in the subfractions indicated that the metabolic rate of the uterine complex saccharides as follows: hyaluronic acid greater than sulfated glycopeptide greater than heparan sulfate greater than chondroitin sulfate C greater than dermatan sulfate. In addition, metabolic heterogeneity of heparan sulfate, chondroitin sulfate A, chondroitin sulfate C, and dermatan sulfate was suggested.

摘要

从经雌激素处理的兔子身上获取子宫切片，将其与N-乙酰-D-[1-³H]葡糖胺以及D-[U-¹⁴C]葡萄糖一起进行体外培养。然后，通过链霉蛋白酶消化、Dowex 1柱色谱法以及在醋酸纤维素膜上进行制备电泳，依次分离出同位素标记的酸性复合糖类。通过这种方式，将各个酸性复合糖类（透明质酸、硫酸乙酰肝素、硫酸软骨素A、硫酸软骨素C、硫酸皮肤素、硫酸糖肽和唾液酸糖肽）分离成2至5个亚组分。亚组分中己糖胺的比放射性表明子宫复合糖类的代谢速率如下：透明质酸＞硫酸糖肽＞硫酸乙酰肝素＞硫酸软骨素C＞硫酸皮肤素。此外，提示硫酸乙酰肝素、硫酸软骨素A、硫酸软骨素C和硫酸皮肤素存在代谢异质性。