Endo M, Yosizawa Z
Biochim Biophys Acta. 1975 Oct 9;404(2):274-80. doi: 10.1016/0304-4165(75)90334-7.
Uterine slices obtained from the estrogen-treated rabbits were digested with pronase. Glycosaminoglycans and acidic glycopeptides were then isolated by Dowex 1 column chromatography and preparative electrophoresis on cellulose acetate membrane (Separax), in succession. Each subfraction thus obtained was identified by the mobility on Separax electrophoresis and the digestibility with mucopolysaccharidases (Streptomyces hyaluronidase, testicular hyaluronidase, chondroitinase AC, chondroitinase ABC and heparinase). The resulting data showed that each complex saccharide (hyaluronic acid, heparan sulfate, chondroitin sulfate A, chondroitin sulfate C, dermatan sulfate, sulfated glycopeptide and sialoglycopeptide) was separated into 2-5 fractions, indicating charge and/or molecular heterogeneity of each complex saccharide.
从经雌激素处理的兔子身上获取子宫切片，用链霉蛋白酶进行消化。然后依次通过Dowex 1柱色谱法和在醋酸纤维素膜（Separax）上进行制备电泳，分离出糖胺聚糖和酸性糖肽。由此获得的每个亚组分通过在Separax电泳上的迁移率以及对粘多糖酶（透明质酸酶、睾丸透明质酸酶、软骨素酶AC、软骨素酶ABC和肝素酶）的消化率来鉴定。所得数据表明，每种复合糖（透明质酸、硫酸乙酰肝素、硫酸软骨素A、硫酸软骨素C、硫酸皮肤素、硫酸化糖肽和唾液酸糖肽）被分离成2至5个组分，表明每种复合糖存在电荷和/或分子异质性。
