Suryanarayana S, von Zastrow M, Kobilka B K
Howard Hughes Medical Institute, Stanford University Medical Center, California 94305.
J Biol Chem. 1992 Nov 5;267(31):21991-4.
Adrenergic receptors are representative of a large family of plasma membrane receptors that interact with G proteins during the process of transmembrane signal transduction. G protein-coupled receptors have a primary structure that is homologous to bacteriorhodopsin and are proposed to have a similar three-dimensional structure; however, it has not yet been possible to examine this hypothesis experimentally. We have used a novel mutagenesis approach to identify intramolecular interactions. Our results indicate that specific amino acids in the seventh hydrophobic segment of alpha 2 and beta 2 adrenergic receptors lie adjacent to the first hydrophobic segment. These studies provide the first experimental evidence defining spatial relationships that exist in the three-dimensional structure of adrenergic receptors.
肾上腺素能受体是一大类质膜受体的代表，它们在跨膜信号转导过程中与G蛋白相互作用。G蛋白偶联受体具有与细菌视紫红质同源的一级结构，并被认为具有相似的三维结构；然而，目前尚无法通过实验检验这一假设。我们采用了一种新的诱变方法来鉴定分子内相互作用。我们的结果表明，α2和β2肾上腺素能受体第七个疏水片段中的特定氨基酸与第一个疏水片段相邻。这些研究提供了首个实验证据，确定了肾上腺素能受体三维结构中存在的空间关系。
