de Bruijn J D, Klein C P, de Groot K, van Blitterswijk C A
Laboratory for Otobiology and Biocompatibility, University of Leiden, The Netherlands.
J Biomed Mater Res. 1992 Oct;26(10):1365-82. doi: 10.1002/jbm.820261008.
Rat bone marrow cells were cultured on plasma-sprayed hydroxyapatite (HA). The cells formed a mineralized extracellular matrix (ECM) that exhibited several characteristics of bone tissue. The interface between this mineralized ECM and the HA was studied at the ultrastructural level with scanning and transmission electron microscopy and x-ray microanalysis. Initially, the deposition of a globular, afibrillar matrix was observed on HA. This was followed by the integration of collagen fibers in this matrix and their subsequent mineralization. At the bone-HA interface two distinctly different interfacial structures were observed. An electron-dense layer with a thickness of 20-60 nm was regularly present, which contained both organic and inorganic material and was rich in glycosaminoglycans. The interfaces differed however, in the presence or absence of an amorphous zone which was free of collagen fibers and had an average thickness of 0.7-0.8 microns. It was frequently seen interposed between the electron-dense layer and the hydroxyapatite. Similar interfacial structures have also been described in the in vivo environment, where they were referred to as lamina limitans-like or cement linelike. From the results of this study, it can be concluded that the described in vitro system is a suitable model to study bone-biomaterial interactions.
将大鼠骨髓细胞培养在等离子喷涂的羟基磷灰石（HA）上。细胞形成了一种矿化的细胞外基质（ECM），该基质呈现出骨组织的几种特征。利用扫描电子显微镜、透射电子显微镜和X射线微分析在超微结构水平上研究了这种矿化ECM与HA之间的界面。最初，在HA上观察到球状、无纤维基质的沉积。随后是胶原纤维在该基质中的整合及其随后的矿化。在骨-HA界面观察到两种明显不同的界面结构。经常出现一层厚度为20-60nm的电子致密层，其包含有机和无机物质，并且富含糖胺聚糖。然而，界面在有无无定形区方面存在差异，该无定形区不含胶原纤维，平均厚度为0.7-0.8微米。它经常夹在电子致密层和羟基磷灰石之间。在体内环境中也描述了类似的界面结构，在那里它们被称为类透明板或类黏固线。从这项研究的结果可以得出结论，所描述的体外系统是研究骨-生物材料相互作用的合适模型。
