Arzt E, Stelzer G, Renner U, Lange M, Müller O A, Stalla G K
Max-Planck-Institute of Psychiatry, Clinical Institute, Munich, Federal Republic of Germany.
J Clin Invest. 1992 Nov;90(5):1944-51. doi: 10.1172/JCI116072.
The production of IL-1 and IL-6 by pituitary cells has recently been demonstrated. In this study we investigated the expression of IL-2 and its receptor (IL-2R) by pituitary cells of different species. In Northern blots, a single hybridizing band of 1 kb, identical to that in normal stimulated lymphocytes, was obtained with specific IL-2 probes. In the mouse AT-20 pituitary tumor cell line, IL-2 mRNA expression was detected after stimulation with corticotropin-releasing hormone or phorbol myristate acetate. In human corticotrophic adenoma cells, basal IL-2 mRNA expression as well as IL-2 secretion were further stimulated by phorbol myristate acetate. Both adenoma and AtT-20 cells showed detectable amounts of IL-2R mRNA and by immunofluorescence, IL-2R membrane expression. In addition, dual immunofluorescence studies in rat anterior pituitary cells demonstrated colocalization of IL-2R with ACTH-positive cells and other cell types expressing the receptor. In addition to the action of lymphocyte-produced IL-2, this cytokine may have a paracrine or autocrine regulatory role within the pituitary. It remains to be established whether IL-2 production occurs in the normal pituitary or is intrinsic to the process of tumor development of these cells. IL-2 may be involved in the growth control of pituitary cells.
最近已证实垂体细胞可产生白细胞介素 -1（IL-1）和白细胞介素 -6（IL-6）。在本研究中，我们调查了不同物种垂体细胞中白细胞介素 -2（IL-2）及其受体（IL-2R）的表达情况。在Northern印迹法中，用特异性IL-2探针获得了一条1 kb的单一杂交带，与正常刺激淋巴细胞中的杂交带相同。在小鼠AT-20垂体肿瘤细胞系中，用促肾上腺皮质激素释放激素或佛波酯刺激后可检测到IL-2 mRNA表达。在人促肾上腺皮质激素腺瘤细胞中，佛波酯进一步刺激了基础IL-2 mRNA表达以及IL-2分泌。腺瘤细胞和AtT-20细胞均显示出可检测到的IL-2R mRNA量，并且通过免疫荧光检测到IL-2R膜表达。此外，对大鼠垂体前叶细胞的双重免疫荧光研究表明，IL-2R与促肾上腺皮质激素（ACTH）阳性细胞以及表达该受体的其他细胞类型共定位。除了淋巴细胞产生的IL-2的作用外，这种细胞因子可能在垂体内具有旁分泌或自分泌调节作用。IL-2的产生是发生在正常垂体中还是这些细胞肿瘤发生过程所固有的，仍有待确定。IL-2可能参与垂体细胞的生长调控。
