人白细胞介素-2受体cDNA的分子克隆与表达

Molecular cloning and expression of cDNAs for the human interleukin-2 receptor.

作者信息

Leonard W J, Depper J M, Crabtree G R, Rudikoff S, Pumphrey J, Robb R J, Krönke M, Svetlik P B, Peffer N J, Waldmann T A

出版信息

Nature. 1984;311(5987):626-31. doi: 10.1038/311626a0.

DOI:10.1038/311626a0

PMID:6090948

Abstract

We have purified the human T-cell growth factor (interleukin-2) receptor and have cloned, sequenced and expressed cDNAs corresponding to this receptor. We identify one gene, but two interleukin-2 receptor mRNAs which differ in their polyadenylation signals. We have isolated an additional cDNA that may correspond to an alternatively spliced mRNA that lacks a 216 base segment and appears to encode an altered membrane protein which cannot bind interleukin-2.

摘要

我们已经纯化了人T细胞生长因子（白细胞介素-2）受体，并克隆、测序并表达了与该受体对应的cDNA。我们鉴定出一个基因，但有两种白细胞介素-2受体mRNA，它们的聚腺苷酸化信号不同。我们还分离出了另一种cDNA，它可能对应于一种选择性剪接的mRNA，该mRNA缺少一个216个碱基的片段，似乎编码一种不能结合白细胞介素-2的改变的膜蛋白。