Sharp G J, Pettitt T R, Rowley A F, Secombes C J
Department of Zoology, University of Aberdeen, Scotland.
J Leukoc Biol. 1992 Feb;51(2):140-5. doi: 10.1002/jlb.51.2.140.
The migration-inducing abilities of both synthetic lipoxin A4 (LXA4) and leukotriene B4 (LTB4) for rainbow trout neutrophils were examined with an in vitro assay. LXA4 caused enhanced migration of these cells with a three- to fourfold greater response than that observed toward LTB4 at most concentrations tested. Checkerboard assays showed that synthetic LXA4 was chemokinetic for trout leukocytes, while LTB4 was chemotactic. The chemokinetic ability of LX synthesized by rainbow trout macrophages maintained in short-term culture was also determined. These cells produced both 4-and 5-series LX, namely LXA4, LXA5, 11-trans-LXA4, 11-trans-LXA5, 7-cis-11-trans-LXA4 and 6(S)-LXA4. Although greater migration was found to LXA4 than to most of the other 4-series positional isomers, at least at some concentrations, this did not exhibit the degree of stereospecificity previously reported in some studies for mammalian leukocytes. Little difference was found between the chemokinetic responses of rainbow trout leukocytes to 4- and 5-series LX. These findings suggest that LXs are important migration-inducing molecules in trout and have a relatively long evolutionary history.
采用体外试验检测了合成脂氧素A4（LXA4）和白三烯B4（LTB4）对虹鳟中性粒细胞的迁移诱导能力。在大多数测试浓度下，LXA4能增强这些细胞的迁移，其反应比LTB4高出三到四倍。棋盘试验表明，合成LXA4对鳟鱼白细胞具有化学促动作用，而LTB4具有趋化作用。还测定了短期培养的虹鳟巨噬细胞合成的LX的化学促动能力。这些细胞产生4-系列和5-系列LX，即LXA4、LXA5、11-反式-LXA4、11-反式-LXA5、7-顺式-11-反式-LXA4和6(S)-LXA4。尽管发现LXA4比大多数其他4-系列位置异构体能引起更大的迁移，至少在某些浓度下是这样，但这并未表现出先前一些研究中报道的哺乳动物白细胞的立体特异性程度。虹鳟白细胞对4-系列和5-系列LX的化学促动反应几乎没有差异。这些发现表明，LX是鳟鱼中重要的迁移诱导分子，并且具有相对较长的进化历史。
