Knight J, Lloyd-Evans P, Rowley A F, Barrow S E
School of Biological Sciences, University College of Swansea, U.K.
J Leukoc Biol. 1993 Dec;54(6):518-22. doi: 10.1002/jlb.54.6.518.
Rainbow trout (Oncorhynchus mykiss) macrophages generated lipoxin (LX) A4, LXA5, leukotriene (LT) B4, LTB5 and 12-hydroxyeicosatetraenoic acid (12-HETE) during the phagocytosis of zymosan and Escherichia coli, but not of the yeast Saccharomyces cerevisiae. Prostaglandin (PG) E2 was also detected in supernatants from macrophages incubated with either zymosan or calcium ionophore A23187. LXA4 (10(-8)-10(-6) M) and LTB4 (10(-9)-10(-7) M) provoked rapid and transient dose-dependent increases in intracellular calcium ([Ca]i) concentrations in leukocyte suspensions containing 40-60% macrophages. EC50 values were 14.9 and 1.2 nM, respectively. PGE2 and 12-HETE had no effect on [Ca]i at concentrations up to 30 microM. PGE2 and 12-HETE (10(-5)-10(-10) M) enhanced the in vitro phagocytosis of yeast test particles by trout macrophages, whereas LXA4 and LTB4 had no demonstrable effect on the responses of these cells at concentrations up to 10(-5) M. In conclusion, the processes involved in trout macrophage stimulation are complex but involve generation of both cyclooxygenase and lipoxygenase products. The increase in [Ca]i caused by LXA4 and LTB4 may form part of the chemotactic transduction mechanism that recruits granulocytes and macrophages to sites of inflammation. The effects of eicosanoids on phagocytosis appear to be independent of changes in [Ca]i.
虹鳟（Oncorhynchus mykiss）巨噬细胞在吞噬酵母聚糖和大肠杆菌过程中会产生脂氧素（LX）A4、LXA5、白三烯（LT）B4、LTB5和12-羟基二十碳四烯酸（12-HETE），但吞噬酿酒酵母时不会产生。在与酵母聚糖或钙离子载体A23187一起孵育的巨噬细胞的上清液中也检测到了前列腺素（PG）E2。LXA4（10⁻⁸ - 10⁻⁶ M）和LTB4（10⁻⁹ - 10⁻⁷ M）可使含有40 - 60%巨噬细胞的白细胞悬液中的细胞内钙（[Ca]i）浓度迅速且短暂地呈剂量依赖性增加。半数有效浓度（EC50）值分别为14.9和1.2 nM。在浓度高达30 μM时，PGE2和12-HETE对[Ca]i没有影响。PGE2和12-HETE（10⁻⁵ - 10⁻¹⁰ M）增强了虹鳟巨噬细胞对酵母测试颗粒的体外吞噬作用，而在浓度高达10⁻⁵ M时，LXA4和LTB4对这些细胞的反应没有明显影响。总之，虹鳟巨噬细胞刺激过程复杂，但涉及环氧化酶和脂氧合酶产物的生成。LXA4和LTB4引起的[Ca]i增加可能是将粒细胞和巨噬细胞募集到炎症部位的趋化转导机制的一部分。类花生酸对吞噬作用的影响似乎与[Ca]i的变化无关。
